Biodiversity Science ›› 2005, Vol. 13 ›› Issue (2): 105-111.doi: 10.1360/biodiv.040179

• Editorial • Previous Article     Next Article

Genetic diversity of Paeonia delavayi as revealed by ISSRs

Shuda Yang1, 2, Suhua Shi, Xun Gong1, 3*, Renchao Zhou3   

  1. 1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
    2 Graduate School of the Chinese Academy of Sciences, Beijing 100039
    3 School of Life Sciences, Sun Yat-sen University, Guangzhou 510275
  • Received:2004-11-22 Revised:2004-12-17 Online:2005-03-20
  • Xun Gong

The genetic diversity of Paeonia delavayi, a species endemic to Southwest China, was analyzed using inter-simple sequence repeat markers (ISSR). A total of 116 bands were amplified from 16 natural populations and one ex situ conserved population by 10 informative and reliable primers of 100 pre-screened primers. Of them, 92 ISSR loci were polymorphic. A relatively high level of intraspecific genetic diversity was revealed: PPB = 44.61%,H = 0.1657 and I = 0.2448 at population level; PPB = 79.31%, H = 0.2947 and I = 0.4355 at species level. The GST = 0.4349 indicated a high degree of genetic differentiation occurred among P. delavayi populations. The results of genetic diversity study, combined with field survey and previous investigations, suggest that P. delavayi is not endangered.

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