Biodiv Sci ›› 2000, Vol. 08 ›› Issue (3): 248-252.DOI: 10.17520/biods.2000034
• 论文 •
MENG Xian-Hong, KONG Jie, ZHUANG Zhi-Meng, WANG Wei-Ji, LIU Ping
Random amplified polymorphic DNA (RAPD) technique was employed to detect the DNA polymorphism in each 23 individuals of the index samples from the wild and hatchery populations of Red
Seabream. A total of 20 decamer arbitrary primers were used and 16 of them generated clear RAPD bands. Of
them , 131 were obtained from the wild population and 123 from the hatchery population. The mean percentage of polymorphic fragments of wild population was 62. 60 %opposite to 54. 47 % in the hatchery population ,
while the average heterozygosity valued 0. 4786 and 0. 3633 , respectively. The richer polymorphic bands and
the higher value of average heterozygosity indicated higher genetic diversity level of this species and the great
potential in piscatorial genetic improvement . The experiment revealed the fact that the percentage of polymorphic fragments and the value of average heterozygosity of the hatchery population were lower than those of the
wild population. As a result , it implied that effective husbandry and management measures must be taken to
avoid reduction of genetic diversity so as to enable the sustainable development of the mariculture.
MENG Xian-Hong, KONG Jie, ZHUANG Zhi-Meng, WANG Wei-Ji, LIU Ping. Genetic diversity in the wild and hatchery populations of Red Seabream (Pagrus major)[J]. Biodiv Sci, 2000, 08(3): 248-252.
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