Biodiv Sci ›› 2000, Vol. 08 ›› Issue (3): 248-252.DOI: 10.17520/biods.2000034

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Genetic diversity in the wild and hatchery populations of Red Seabream (Pagrus major)

MENG Xian-Hong, KONG Jie, ZHUANG Zhi-Meng, WANG Wei-Ji, LIU Ping   

  1. 1) Ocean University of Qingdao , Qingdao  266003
    2) Yellow Sea Fisheries Research Institute , Qingdao  266071
  • Received:1999-11-08 Revised:2000-06-23 Online:2000-08-20 Published:2000-08-20
  • Contact: MENG Xian-Hong

Abstract: Random amplified polymorphic DNA (RAPD) technique was employed to detect the DNA polymorphism in each 23 individuals of the index samples from the wild and hatchery populations of Red Seabream. A total of 20 decamer arbitrary primers were used and 16 of them generated clear RAPD bands. Of them , 131 were obtained from the wild population and 123 from the hatchery population. The mean percentage of polymorphic fragments of wild population was 62. 60 %opposite to 54. 47 % in the hatchery population , while the average heterozygosity valued 0. 4786 and 0. 3633 , respectively. The richer polymorphic bands and the higher value of average heterozygosity indicated higher genetic diversity level of this species and the great potential in piscatorial genetic improvement . The experiment revealed the fact that the percentage of polymorphic fragments and the value of average heterozygosity of the hatchery population were lower than those of the wild population. As a result , it implied that effective husbandry and management measures must be taken to avoid reduction of genetic diversity so as to enable the sustainable development of the mariculture.