Biodiversity Science ›› 2000, Vol. 08 ›› Issue (3): 248-252.doi: 10.17520/biods.2000034

• 论文 • Previous Article     Next Article

Genetic diversity in the wild and hatchery populations of Red Seabream (Pagrus major)

MENG Xian-Hong, KONG Jie, ZHUANG Zhi-Meng, WANG Wei-Ji, LIU Ping   

  1. 1) Ocean University of Qingdao , Qingdao  266003
    2) Yellow Sea Fisheries Research Institute , Qingdao  266071
  • Received:1999-11-08 Revised:2000-06-23 Online:2000-08-20
  • MENG Xian-Hong

Random amplified polymorphic DNA (RAPD) technique was employed to detect the DNA polymorphism in each 23 individuals of the index samples from the wild and hatchery populations of Red Seabream. A total of 20 decamer arbitrary primers were used and 16 of them generated clear RAPD bands. Of them , 131 were obtained from the wild population and 123 from the hatchery population. The mean percentage of polymorphic fragments of wild population was 62. 60 %opposite to 54. 47 % in the hatchery population , while the average heterozygosity valued 0. 4786 and 0. 3633 , respectively. The richer polymorphic bands and the higher value of average heterozygosity indicated higher genetic diversity level of this species and the great potential in piscatorial genetic improvement . The experiment revealed the fact that the percentage of polymorphic fragments and the value of average heterozygosity of the hatchery population were lower than those of the wild population. As a result , it implied that effective husbandry and management measures must be taken to avoid reduction of genetic diversity so as to enable the sustainable development of the mariculture.

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