Biodiversity Science ›› 2003, Vol. 11 ›› Issue (1): 10-19.doi: 10.17520/biods.2003002

• 论文 • Previous Article     Next Article

Molecular phylogenetic analysis of Trichoderma harzianum and its related species

ZHANG Chu Long, XU Tong*   

  1. 1 Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029
    2 Systematic Mycology & Lichenology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080
  • Received:2002-03-19 Revised:2002-06-22 Online:2003-01-20
  • XU Tong

Trichoderma harzianum is one of the most common aggregate species of the genus. Based on different molecular characterizations, strains of T. harzianum have been divided into different subgroups, which has created confusion. We reexamined reference strains representing different subgroups under the name T. harzianum and its related species. Phylogenetic analysis using the NEIGHBOR program in the PHYLIP package based on ITS1 5.8S rDNA ITS2 sequence data distributed the tested strains among two phylogenetic groups (A and B), which was supported by high bootstrap values. Group A contains T. hamatum , T.asperellum, T. atroviride, T.koningii and T.viride , and is divided into two clades. T.viride is more closely related to T.koningii and T.atroviride than to T. hamatum and T. asperellum . Group B contains T. spirale , T. hamatum , T. inhamatum , T. harzianum and T. anam. Hypocrea vinosa , and is divided into six clades. T. inhamatum is further divided into two subgroups (Ti1 and Ti2), and T. harzianum comprises at least five subgroups(Th1, Th2, Th4, Th5 and Th6). The phylogenetic analysis of ITS1 5.8S rDNA ITS2 sequence also indicated the heterogeneity of T. hamatum ,since a neotype of T. hamatum is located in group A but other strains of T. hamatum are located in group B. The RAPD analysis of 18 strains representing T. harzianum and its related species coincided with phylogeny based on sequence analysis of the ITS regions.

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[1] CHANG Zhao-Yang*, WU Zhen-Hai, XU Lang-Ran. Onobrychis micrantha Schrenk, a newly recorded species of Leguminosae from China[J]. J Syst Evol, 2006, 44(2): 187 -188 .
[2] De-Xing Zhang. Unorthodox reflections on molecular ecology research in China[J]. Biodiv Sci, 2015, 23(5): 559 -569 .
[3] TONG HAOWEN. Continuing Diversifying the only Megatrend of Evolution[J]. Biodiv Sci, 1995, 03(Suppl.): 94 -96 .
[4] Yang Zi-Xiang, Xi Sheng-Ke. A Study on Isozymes of Peroxidase of 10 Species in Juglans L.[J]. J Syst Evol, 1989, 27(1): 53 -57 .
[5] ZHOU Qing;YANG Jing;SHAO Ai-Hua and WANG Ya-Ling. Effect of NaHSO3 on Root Growth and The Physiological Characteristics in Rice Seedling[J]. Chin Bull Bot, 1998, 15(03): 51 -53 .
[6] Paulo C. Baleeiro, Richard W. Jobson, Paulo T. Sano. Morphometric approach to address taxonomic problems: The case of Utricularia sect. Foliosa (Lentibulariaceae)[J]. J Syst Evol, 2016, 54(2): 175 -186 .
[7] Xia Yi-shen and Shao Tin-fu. Advances in Study on Physiology and Biochemistry of Fruit Ripening[J]. Chin Bull Bot, 1989, 6(01): 5 -8 .
[8] Nan Liu, Lanlan Guan. Linkages between woody plant proliferation dynamics and plant physiological traits in southwestern North America[J]. J Plant Ecol, 2012, 5(4): 407 -416 .
[9] YANG Han-Qi-, Sun-Mao-Sheng. Supplementary Description of Inflorescences and Fruits for Dendrocalamus asper and D.barbatus (Poaceae: Bambusoideae)[J]. Plant Diversity, 2011, 33(2): 161 -163 .
[10] Xie Shou-chang, Liu Wen-yue, Li Shou-chang, Yang Guo-ping. Preliminary Studies on the Biomass of Middle-Mountain Moist Evergreen Broadleaved Forests in Ailao Mountain, Yunnan[J]. Chin J Plan Ecolo, 1996, 20(2): 167 -176 .