Biodiversity Science ›› 1999, Vol. 07 ›› Issue (4): 277-284.doi: 10.17520/biods.1999043

• 论文 • Previous Article     Next Article

Characterization of soybean rhizobia at different levels using PCR based techniques


  1. 1 Department of Microbiology,Huazhong Agricultural University,Wuhan 430070,China
    2 Department of Microbiology,Wageningen Agricultural University,Wageningen,The Netherslands
  • Online:1999-11-20

Nineteen standard USDA strains reprenting three recognized soybean rhizobia,Sinorhizobium fredii,Bradyrhizobium japonicum and B.elkanii,were examined by restrietion fragment length Polymorphism (RFLP) analysis of 16S rRNA genes amplified by Polymerase chain reactin (PCR).Four composite genotypes were obtained from the combined data of the RFLP analysis with three endonucleases,S.fredii,Bjaponicum,Belkanii Ⅱ and Ⅱa reference strains fell into these four genotypes respectively.No genotype was shared by two speciies.Therefore,16SrDNA PCR-RFLP is a rapid tool for the identification of soybean rhizobia.According to 16SrDNA PCR-FRLP,22 fast-growing and 19 slow-growing soybean rhizobia strains form China were identified to S.fredii and B.japonicum,respectively. The reference strains and wild type isolates were also examined by 16S~23S intergenic spacer PCRE-RFLP analysis.PCR results indicated that the length of the intergenic region between S.fredii and Bradyrhizobium different.All fast-growing S.fredii strains produced one large band (2.1kb),and all slow-growing Bradyrhi-zobium strains produced one smaller band (2.0kb).Based on 16S~23SRFLP analysis,22S.fredii isolate can be further separated into two "genotypes"that originated from two different regions in China;19B.japonicum were also characterized at strain level by REP (repetitive extragenic palindromic)and ERIC (enterobacterial repetitive intergenic consensus)PCR fingerprinting.

No related articles found!
Full text



[1] ZENG Yi, XIA Nian-He, LIN Ru-Shun. A New Species of Gigantochloa Kurz ex Munro (Poaceae: Bambusoideae) from Yunnan Province, China[J]. Plant Diversity, 2014, 36(05): 581 -583 .
[2] Yihao Shi, Jiaying Huang, Tianshu Sun, Xuefei Wang, Chenqi Zhu, Yuxi Ai and Hongya Gu. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana[J]. J Integr Plant Biol, 2017, 59(2): 118 -133 .
[3] Xiufen Che, Xiaobo Yang, Ping Yue, Zhiyang Ou, Donghai Li, Qingshu Wu. Species diversity of forests in Tongguling National Nature Reserve, Hainan[J]. Biodiv Sci, 2006, 14(4): 292 -299 .
[4] Zhang Jingwei. Mallee——A Special Vegetation Type in Australia[J]. Chin J Plan Ecolo, 1983, 7(3): 256 -259 .
[5] ZHANG Cheng Yi, YU Fei Hai, DONG Ming. Effects of Sand Burial on the Survival, Growth, and Biomass Allocation in Semi-shrub Hedysarum laeve Seedlings[J]. J Integr Plant Biol, 2002, 44(3): 337 -343 .
[6] . [J]. Chin Bull Bot, 1994, 11(专辑): 76 .
[7] Ali Irfan ILBAS, Yasemin EROGLU and Halil Erhan EROGLU. Effects of the Application of Different Concentrations of NaN3 for Different Times on the Morphological and Cytogenetic Characteristics of Barley (Hordeum vulgare L.) Seedlings[J]. J Integr Plant Biol, 2005, 47(9): .
[8] GONG Ji-Rui, HUANG Yong-Mei, GE Zhi-Wei, DUAN Qing-Wei, YOU Xin, AN Ran, ZHANG Xin-Shi. ECOLOGICAL RESPONSES TO SOIL WATER CONTENT IN FOUR HYBRID POPULUS CLONES[J]. Chin J Plan Ecolo, 2009, 33(2): 387 -396 .
[9] HUANG Ben-Hong. Late Paleozoic Flora in Nei Mongol Plateau[J]. Chin Bull Bot, 2000, 17(专辑): 172 -178 .
[10] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .