生物多样性

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野生罗汉果遗传多样性的ISSR分析

彭云滔1,唐绍清1*,李伯林1,刘燕华2   

  1. 1 (广西师范大学生命科学学院,桂林541004)
    2 (广西师范大学附属中学,桂林 541001)
  • 收稿日期:2004-05-18 修回日期:2004-08-25 出版日期:2005-01-20 发布日期:2005-01-20
  • 通讯作者: 唐绍清

Genetic diversity of Siraitia grosvenorii detected by ISSR markers

Yuntao Peng1, Shaoqing Tang1, Bolin Li1, Yanhua Liu2   

  1. 1 College of Life Science, Guangxi Normal University, Guilin 541004
    2 The High School Affiliated to Guangxi Normal University,Guilin 541001
  • Received:2004-05-18 Revised:2004-08-25 Online:2005-01-20 Published:2005-01-20
  • Contact: Shaoqing Tang

摘要:

应用ISSR分子标记方法对采自广西和广东的7个罗汉果(Siraitia grosvenorii)野生居群共130个个体进行了遗传多样性分析。15个ISSR引物共扩增到了111个位点,其中91个是多态性位点,占82.0%。Nei′s基因多样性指数(He)为 0.248,Shannon 信息多样性指数(I) 为0.354。罗汉果不同居群的遗传多样性水平差异较大,居群多态位点百分率在 28.2%-55.6%之间,Nei′s基因多样性指数为0.080-0.209,Shannon 信息多样性指数为0.123-0.310。永福居群(YF)和金秀居群(JX)的遗传多样性水平较高,其周边居群的遗传多样性水平逐渐降低,居群间产生了较大的遗传分化(Gst = 0.569)。居群间的遗传距离与地理距离相关性不明显(r =0.369,P = 0.115)。UPGMA聚类图中,7个居群的个体按居群各自聚在一起。

关键词: 共适应, 远交衰退, 遗传同化, 杂种优势, 渐渗杂交

Abstract:

Siraitia grosvenorii, a liana species of the Cucurbitaceae endemic to southern China, is an important economic plant. The genetic diversity of 130 individuals from seven Siraitia grosvenorii populations from Guangxi and Guangdong provinces was estimated using inter-simple sequence repeat (ISSR) markers. The fifteen primers employed produced a total of 111 scorable amplified fragments, of which 91 (82.0%) are polymorphic. Genetic diversity analysis showed that Nei′s gene diversity (He) was 0.248 and Shannon′s genetic diversity index (I) was 0.354. Genetic differentiation mainly occurred among populations (Gst=0.569). The correlation between genetic distance and geographic distance among the populations was not significant (r = 0.369, P=0.115). UPGMA cluster analysis showed that the 130 individuals were clustered into seven clusters corresponding to the seven populations.

Key words: coadaptation, outbreeding depression, genetic assimilation, heterosis, introgression