生物多样性

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新疆不同来源金黄壳囊孢的多样性

马荣1, 尹永香2, 赵颖1, 张志东3, 田呈明4   

  1. 1. 新疆农业大学林学与园艺学院
    2. 新疆农业大学林业研究所
    3. 新疆农业科学院微生物应用研究所
    4. 北京林业大学省部共建森林培育与保护教育部重点实验室
  • 收稿日期:2019-05-29 修回日期:2019-10-08 出版日期:2019-11-12
  • 通讯作者: 田呈明

The diversity of Cytospora chrysosperma from different hosts in Xinjiang

Rong Ma1, Yongxiang Yin2, Ying Zhao1, Zhidong Zhang3, Chengming Tian   

  • Received:2019-05-29 Revised:2019-10-08 Online:2019-11-12
  • Contact: Chengming Tian

为明确新疆不同寄主及地理来源的金黄壳囊孢的多样性,探讨种内亲缘关系和多样性差异。通过记录菌株在PDA培养基上的菌落颜色、形状、子实体形态等特征,并应用Biolog-FF技术及ISSR分子标记技术,比较了来自于新疆6个地区5种寄主上的47株金黄壳囊孢(Cytospora chrysosperma)的培养特征、生理生化特征及遗传多样性。结果表明47株金黄壳囊孢依据培养特征可划分为15种类型。不同类型菌株在碳源利用及代谢能力上存在差异,各菌株对碳源的利用数量随着培养时间的增长逐渐增多。菌株882利用的碳源种类最多,培养120 h 可利用28种不同碳源,碳源代谢能力中等;菌株812-1利用的碳源数量最少,培养120 h仅利用7种碳源,代谢能力较低;菌株1074-2、 847、 934、891-1、896、740具有单独利用碳源的能力。基于遗传相似系数进行聚类分析,结果显示遗传系数为0.58时,47个菌株被划分为两大类群,其中第二类群菌株的培养特征为:菌落白色、子实体较小且分布密集。供试金黄壳囊孢的多样性主要受自身遗传结构的影响,不同寄主种类和地理来源对多样性的影响不显著。

关键词: 金黄壳囊孢菌, 培养性状, Biolog-FF技术, ISSR分子标记

In order to clarify the diversity, phylogenetic relationship and genetic difference of Cytospora Chrysosperma from different hosts in Xinjiang. The cultural characteristics, Biolog-FF and ISSR molecular marker were used to analyze the physiological-biochemical characteristic and genetic diversity of C. chrysosperma. The results showed that 47 strains of C. chrysosperma have been divided into 15 types based on the culture characteristics. Different strains have different carbon source utilization and metabolic capacity. The amount of carbon source utilization by each strain increases with the growth time. The strain 882 utilized 28 different carbon sources for 120 h culture and the metabolic capacity of carbon sources is moderate. The strain 812-1 only utilized 10 carbon sources for 120 h and its metabolic capacity is low. Strains 740, 1074-2, 891-1, 847, 896 and 934 are able to utilize individual carbon sources. 47 strains of C. chrysosperma were divided into two clusters by cluster analysis based on genetic similarity when the genetic coefficient was 0.58. The second cluster have similar culture characteristics: white clonies, small and dense fruiting bodies. The genetic diversity of C. chrysosperma is mainly effect by its genetic structure. The influence from host species and geographical origins is not significant.

Key words: Cytospora chrysosperma, ISSR, cultural characteristics, Biolog-FF

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