生物多样性 ›› 2004, Vol. 12 ›› Issue (3): 370-376.doi: 10.17520/biods.2004045

• 论文 • 上一篇    下一篇

中国东部栗疫病菌dsRNA群体的多样性

徐陈贤, 刘福秀, 周晓云, 王克荣*   

  1. (南京农业大学植物病理学系,南京 210095)
  • 收稿日期:2003-11-04 修回日期:2004-03-11 出版日期:2004-05-20
  • 通讯作者: 王克荣

Diversity of the dsRNA population of Cryphonectria parasitica from eastern China

XU Chen-Xian, LIU Fu-Xiu, ZHOU Xiao-Yun, WANG Ke-Rong*   

  1. Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095
  • Received:2003-11-04 Revised:2004-03-11 Online:2004-05-20
  • Contact: WANG Ke-Rong

采用生物学测定和凝胶电泳的方法,对中国东部栗疫病菌(Cryphonectria parasitica)的dsRNA病毒进行了研究。根据电泳图谱,中国东部栗疫病菌的dsRNA病毒可以分为5种类型,其中类型I只具有一个12.7 kb的条带,占大多数; 类型II有两条带,一条12.7 kb,另一条5.2 kb左右,只有一个菌株354; 类型III则除了12.7 kb的条带外,还有3条小于2 kb的小片段,有238和250两个菌株;dsRNA属于类型IV的为269和344两个菌株,它只含有两个1.8-3.1 kb的小片段;dsRNA类型V只有一个菌株280,除了一条12.7 kb分子外,还有4条2.6-3.3 kb的小片段。含有dsRNA的菌株培养性状多样,根据菌落特征,栗疫病菌可以划分为5种培养类型,但菌株的培养类型与dsRNA的类型间没有对应的关系。毒力测定表明含有dsRNA病毒的供试菌株大多属于低毒力类型。

Fourtyone dsRNA viruses of Cryphonectria parasitica isolates from eastern China were divided into five types on the basis of gel electrophoresis patterns. Type I contained only one segment of 12.7 kb, accounting for majority of the dsRNA viruses. Type II had another segment about 5.2 kb besides the segment of 12.7 kb, and was only found in the pathogen isolate 354. Type III possessed the 12.7 kb segment and other three small ones shorter than 2 kb, which was detected from the two isolates 238 and 250. Type IV was separated from pathogen isolates 269 and 344, which contained only two small dsRNA segments about 1.8-3.1 kb. Type V, including four small dsRNA segments about 2.6-3.3 kb and the 12.7 kb one, was only extracted from the isolate 280. The cultural characters of all these tested isolates, whether or not including dsRNA viruses, were diverse and could be also divided into five cultural types. The cultural type of C. parasitica isolate is not consistent with the type of dsRNA virus and virulence test results showed that most of the tested isolates containing dsRNA viruses were hypovirulent.

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