A comparison of genetic diversity between wild and cultured populations of the Chinese giant salamander, Andrias davidianus, based on microsatellite analyses

doi:10.3724/SP.J.1003.2008.08105

Abstract

Abstract:

The Chinese giant salamander (Andrias davidianus) is world’s largest amphibian and is endemic to China. Wild populations of the species have declined drastically, while domesticated populations have increased in recent years. To estimate genetic variation in the Chinese giant salamander, we analyzed ten microsatellite loci of 44 individuals from two wild and one domesticated populations. A total of 52 alleles were found from seven loci shown to be polymorphic. The number of alleles ranged from 6 to 9 (mean 7.4). The polymorphism information content (PIC) of all populations at all loci exceeded 0.54 except the YQY at GS134 (0.3750), suggesting high polymorphism at microsatellite markers. Compared with the wild populations, some alleles in the domesticated population were drifted and thePIC was lower. Our results may provide a theoretical basis for conservation and exploitation of giant salamanders in China.

Key words: Andrias davidianus, microsatellite DNA markers, allele, PIC

Yan Meng, Yanqing Yang, Yan Zhang, Hanbing Xiao. A comparison of genetic diversity between wild and cultured populations of the Chinese giant salamander, Andrias davidianus, based on microsatellite analyses[J]. Biodiv Sci, 2008, 16(6): 533-538.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2008.08105

https://www.biodiversity-science.net/EN/Y2008/V16/I6/533

Figures/Tables 3

References 12

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座位 Locus	引物序列 Primer sequence (5'-3')	片段大小 Size (bp)	重复类型 SSR motif	退火温度 Annealing temperature (℃)
GS82	F: CGTAGGGGTTACAAAATCA	227	(AG)₂₀	48.0
GS82	R: CAGTCTGTGCCATTCATTTC	227	(AG)₂₀	48.0
GS17	F: TTCTCATTTTGTTTCCTATTGC	266	(CT)₂₀	48.0
GS17	R: CACAGTAAGGACGCAGTAAAA	266	(CT)₂₀	48.0
GS120	F: TCTCATTTTGTTTCCTATTG	239	(CT)₂₀	54.5
GS120	R: GTTGGAAATTAAAGGCAC	239	(CT)₂₀	54.5
GS134	F: TTCTCACCAATATGCCACC	168	(GA)₁₇	60.8
GS134	R: TCAACTTTGCTGAAAATCCA	168	(GA)₁₇	60.8
GS66	F: GTGATTACAACTCGTGGACTG	205	(GTGA)₂₀	60.8
GS66	R: GTAGTCTTGGTGGGAGGGTAG	205	(GTGA)₂₀	60.8
GS132	F: CATACATCTACAACTACATCCGA	206	(AC)₃₀	59.0
GS132	R: TCTTCAAGCGAGCTTTTACT	206	(AC)₃₀	59.0
GS96	F: ACAGTAAGGACGCAGTAAA	163	(AG)₂₀	60.0
GS96	R: TCTAACCTACCACTCCTGCT	163	(AG)₂₀	60.0
GS78	F: ATTGGGGAGAAATAAAGTG	219	(TAGA)₂₀	53.0
GS78	R: GTGCTTGCACAACCTAATC	219	(TAGA)₂₀	53.0
GS105	F: CTTACATTTCGCACGGATTT	260	(AG)₅…(AG)₆	56.5
GS105	R: ATGGCACATTGTAGGAGTTT	260	(AG)₅…(AG)₆	56.5
GS122	F: TTCAGGAGGGACAGGGAGA	370	(AG)₁₀…(AG)₅…(AG)₅…(AG)₅…(AG)₁₂	59.6
GS122	R: TGCCCTTCTTTAGAGCTACTTCC	370	(AG)₁₀…(AG)₅…(AG)₅…(AG)₅…(AG)₁₂	59.6

座位 Locus	引物序列 Primer sequence (5'-3')	片段大小 Size (bp)	重复类型 SSR motif	退火温度 Annealing temperature (℃)
GS82	F: CGTAGGGGTTACAAAATCA	227	(AG)₂₀	48.0
GS82	R: CAGTCTGTGCCATTCATTTC	227	(AG)₂₀	48.0
GS17	F: TTCTCATTTTGTTTCCTATTGC	266	(CT)₂₀	48.0
GS17	R: CACAGTAAGGACGCAGTAAAA	266	(CT)₂₀	48.0
GS120	F: TCTCATTTTGTTTCCTATTG	239	(CT)₂₀	54.5
GS120	R: GTTGGAAATTAAAGGCAC	239	(CT)₂₀	54.5
GS134	F: TTCTCACCAATATGCCACC	168	(GA)₁₇	60.8
GS134	R: TCAACTTTGCTGAAAATCCA	168	(GA)₁₇	60.8
GS66	F: GTGATTACAACTCGTGGACTG	205	(GTGA)₂₀	60.8
GS66	R: GTAGTCTTGGTGGGAGGGTAG	205	(GTGA)₂₀	60.8
GS132	F: CATACATCTACAACTACATCCGA	206	(AC)₃₀	59.0
GS132	R: TCTTCAAGCGAGCTTTTACT	206	(AC)₃₀	59.0
GS96	F: ACAGTAAGGACGCAGTAAA	163	(AG)₂₀	60.0
GS96	R: TCTAACCTACCACTCCTGCT	163	(AG)₂₀	60.0
GS78	F: ATTGGGGAGAAATAAAGTG	219	(TAGA)₂₀	53.0
GS78	R: GTGCTTGCACAACCTAATC	219	(TAGA)₂₀	53.0
GS105	F: CTTACATTTCGCACGGATTT	260	(AG)₅…(AG)₆	56.5
GS105	R: ATGGCACATTGTAGGAGTTT	260	(AG)₅…(AG)₆	56.5
GS122	F: TTCAGGAGGGACAGGGAGA	370	(AG)₁₀…(AG)₅…(AG)₅…(AG)₅…(AG)₁₂	59.6
GS122	R: TGCCCTTCTTTAGAGCTACTTCC	370	(AG)₁₀…(AG)₅…(AG)₅…(AG)₅…(AG)₁₂	59.6

座位 Locus	等位基因 Allele	群体 Population			座位 Locus	等位基因 Allele	群体 Population
座位 Locus	等位基因 Allele	CJY	YQY	YQR			CJY	YQY	YQR
GS122	A	0.2188	0.2000	0.2000	GS132	A	0.1667	0.1500	0.1667
	B	0.1875	0.2500	0.3000		B	0.0833	0.1000	0.2778
	C	0.0938	0.0500	-		C	0.1389	-	-
	D	0.2188	0.3000	0.2500		D	0.2500	0.2500	0.1667
	E	0.1250	0.1500	0.2500		E	-	0.1000	-
	F	0.1562	0.0500	-		F	-	0.1000	-
GS134	A	0.0556	0.1875	0.0625		G	0.1111	0.1000	0.2778
	B	0.0833	0.1875	0.3750		H	0.1944	0.1000	0.1111
	C	0.2778	0.1250	0.1875		I	0.0556	0.1000	-
	D	0.1389	-	-	GS105	A	-	0.1000	-
	E	0.0278	0.1250	-		B	-	0.0500	0.0500
	F	0.3056	0.3750	0.0625		C	0.3529	0.2500	0.3500
	G	0.1111	-	0.3125		D	0.1176	0.0500	0.1000
GS66	A	0.0625	-	0.1000		E	0.0294	-	-
	B	0.2188	0.1111	0.1500		F	-	0.1500	-
	C	0.2188	0.1667	0.0500		G	0.0588	0.0500	-
	D	0.2188	0.1667	0.0500		H	0.4118	0.3000	-
	E	0.2188	0.1111	0.4000		I	0.0294	0.0500	0.5000
	F	0.0625	0.2222	0.2500	GS78	A	0.0278	0.1111	-
	G	0.0938	0.1667	-		B	0.0556	0.0556	0.1250
	H	-	0.0556	-		C	0.2778	0.0556	0.0625
GS96	A	0.1667	0.2222	-		D	0.3889	0.1111	-
	B	-	0.1667	0.4444		E	0.1111	0.5556	0.5625
	C	0.1667	0.2222	0.0556		F	0.1111	-	0.2500
	D	0.4444	0.2778	0.1667		G	0.0278	0.1111	-
	E	0.1389	0.1111	0.3333
	F	0.0833	-	-

座位 Locus	等位基因 Allele	群体 Population			座位 Locus	等位基因 Allele	群体 Population
座位 Locus	等位基因 Allele	CJY	YQY	YQR			CJY	YQY	YQR
GS122	A	0.2188	0.2000	0.2000	GS132	A	0.1667	0.1500	0.1667
	B	0.1875	0.2500	0.3000		B	0.0833	0.1000	0.2778
	C	0.0938	0.0500	-		C	0.1389	-	-
	D	0.2188	0.3000	0.2500		D	0.2500	0.2500	0.1667
	E	0.1250	0.1500	0.2500		E	-	0.1000	-
	F	0.1562	0.0500	-		F	-	0.1000	-
GS134	A	0.0556	0.1875	0.0625		G	0.1111	0.1000	0.2778
	B	0.0833	0.1875	0.3750		H	0.1944	0.1000	0.1111
	C	0.2778	0.1250	0.1875		I	0.0556	0.1000	-
	D	0.1389	-	-	GS105	A	-	0.1000	-
	E	0.0278	0.1250	-		B	-	0.0500	0.0500
	F	0.3056	0.3750	0.0625		C	0.3529	0.2500	0.3500
	G	0.1111	-	0.3125		D	0.1176	0.0500	0.1000
GS66	A	0.0625	-	0.1000		E	0.0294	-	-
	B	0.2188	0.1111	0.1500		F	-	0.1500	-
	C	0.2188	0.1667	0.0500		G	0.0588	0.0500	-
	D	0.2188	0.1667	0.0500		H	0.4118	0.3000	-
	E	0.2188	0.1111	0.4000		I	0.0294	0.0500	0.5000
	F	0.0625	0.2222	0.2500	GS78	A	0.0278	0.1111	-
	G	0.0938	0.1667	-		B	0.0556	0.0556	0.1250
	H	-	0.0556	-		C	0.2778	0.0556	0.0625
GS96	A	0.1667	0.2222	-		D	0.3889	0.1111	-
	B	-	0.1667	0.4444		E	0.1111	0.5556	0.5625
	C	0.1667	0.2222	0.0556		F	0.1111	-	0.2500
	D	0.4444	0.2778	0.1667		G	0.0278	0.1111	-
	E	0.1389	0.1111	0.3333
	F	0.0833	-	-

位点 Locus	群体 Population	等位基因数 N_a	有效等位基因数 N_e	Shannon 指数 I	多态信息含量 PIC	固定指数 F	观察杂合度 H_o	期望杂合度 H_e	平均杂合度 Heterozygosity
GS96	CJY	6	3.5801	1.4389	0.6837	-0.2740	0.9444	0.7413	0.7207
	YQY	6	4.6286	1.5671	0.7490	-0.0708	0.8889	0.8301	0.7840
	YQR	4	3.5433	1.3101	0.5974	-0.3468	1.0000	0.7425	0.7178
GS122	CJY	6	5.5652	1.7507	0.7947	-0.1809	1.0000	0.8468	0.8203
	YQY	6	4.5455	1.6138	0.7457	-0.2179	1.0000	0.8211	0.7800
	YQR	4	3.7190	1.3479	0.6975	-0.3222	1.0000	0.7563	0.7311
GS134	CJY	7	4.6957	1.7036	0.7569	-0.2353	1.0000	0.8095	0.7870
	YQY	5	4.1290	1.5154	0.3750	-0.2372	1.0000	0.8083	0.7578
	YQR	5	3.3800	1.3986	0.6702	-0.3656	1.0000	0.7323	0.7041
GS66	CJY	7	5.6889	1.8258	0.7579	0.6327	0.3125	0.8508	0.8242
	YQY	7	6.2308	1.8790	0.8188	0.1250	0.7778	0.8889	0.8395
	YQR	6	5.0196	1.7671	0.7025	0.1683	0.6875	0.8266	0.8008
GS132	CJY	7	5.8909	1.8496	0.8081	-0.1710	1.0000	0.8540	0.8302
	YQY	8	6.8966	2.0127	0.8390	0.0000	0.9000	0.9000	0.8550
	YQR	5	5.2941	1.8372	0.7819	-0.1918	1.0000	0.8391	0.8111
GS78	CJY	7	3.8802	1.5710	0.8571	0.3451	0.5000	0.7635	0.7423
	YQY	6	2.8421	1.3801	0.6200	0.1904	0.5556	0.6863	0.6481
	YQR	4	3.4386	1.4205	0.5471	0.4172	0.4286	0.7354	0.7092
GS105	CJY	6	3.1934	1.3588	0.6333	-0.4130	1.0000	0.7077	0.6869
	YQY	8	5.1282	1.8217	0.7796	-0.1801	1.0000	0.8474	0.8050
	YQR	4	2.6806	1.1084	0.5444	-0.5451	1.0000	0.6472	0.6270