Abstract: We surveyed the macrofauna diversity at five sampling stations of the Jiaozhou Bay during seven cruises from Sept. 2003 to Sept. 2004. The species diversity was studied based on quantitative investigations of species composition, biomass and secondary production. The results showed the following: stations D and S, located at Dagu River estuary and central channel respectively, had the lowest diversity indices. Diversity indices differed significantly between the stations inside and oustide the aquicultural area. The number of species (S) and Margalef’s richness index (D) demonstrated distinct seasonal variations: they were lower in spring and autumn and higher in winter and summer. Among all the environmental factors examined, only Shannon-Wiener diversity index (H′) and Margalef’s richness index had negative correlations with macro-faunal secondary production. Both the results of diversity indices and abundance/biomass curves indicated that the macrobenthic community in aquicultural areas was disturbed to a certain degree.