生物多样性 ›› 1995, Vol. 03 ›› Issue (1): 1-7.  DOI: 10.17520/biods.1995001

• 论文 •    下一篇

西南地区地方品种猪血液蛋白遗传多样性研究

聂龙,施立明   

  1. (中国科学院昆明动物研究所细胞与分子进化实验室,昆明 650223)
  • 收稿日期:1994-03-03 修回日期:1994-05-11 出版日期:1995-02-20 发布日期:1995-02-20

Genetic relationship among several native pig breeds in Southwest China: An approach from blood pro-tein electrophoresis

Nie Long,Shi Liming   

  1. Laboratory of Cellular and Molecular Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223
  • Received:1994-03-03 Revised:1994-05-11 Online:1995-02-20 Published:1995-02-20

摘要: 本实验采用水平板淀粉凝胶电泳技术,研究滇南小耳猪、迪庆藏猪、明光小耳猪、福贡猪、越南野猪和贵州从江香猪共20个个体共计42个基因座位的蛋白质多态性,发现其基因型和基因频率大部分相似,只有Tf、Hp、EsD、Amy-1、CEs和6PGD等六个座位具有多态性;多态座位百分比和平均杂合度分别为P=0.0714,H=0.051。用UPGMA法分别对由基因频率所计算的标准遗传距离和欧氏几何距离 进行聚类分析,结果表明贵州从江香猪和其他几个云南猪品种(系)的距离最远。

AbstractIn the present study, analysis of 42 gene loci of native pigs in Southwest China were carried out by meansof horizontal starch gel electrophoresis. Genotype and gene frequency were general1y similar, the polymor-phism was found only in Tf. Hp. Amy-1. CEs. 6PGD and EsD.Genetic variability was quantified as follows: the proportion of polymprphism loci(P) and the averageheterozygosity (H) were 0. 0714 and 0. 051, respectively. These values were low comparing to those ofdomestic pigs reported. Using the gene frequencies of the 42 loci, the genetic distance between six popula-tions were calculated by two formulae of Nei and Rogers, and UPGMA dendrogram were made. From thedendrogram, it is clear that Xiang pigs is far distanced from other pigs.