生物多样性 ›› 2010, Vol. 18 ›› Issue (2): 120-128. DOI: 10.3724/SP.J.1003.2010.125
收稿日期:
2009-09-25
接受日期:
2010-03-19
出版日期:
2010-03-20
发布日期:
2010-03-20
通讯作者:
李火根
基金资助:
Received:
2009-09-25
Accepted:
2010-03-19
Online:
2010-03-20
Published:
2010-03-20
Contact:
Huogen Li
摘要:
引进物种常与本土近缘种间发生杂交而导致基因渐渗, 进而影响本地种的遗传系统。鹅掌楸(Liriodendron chinense)为我国濒危树种之一, 鹅掌楸种间杂交可配性高, 近10年来随着北美鹅掌楸(L. tulipifera)的大量引种及杂交鹅掌楸的繁殖推广, 鹅掌楸种间发生基因渐渗的可能性大大增加。本文以5个包含鹅掌楸、北美鹅掌楸、杂交鹅掌楸的人工混交林为实验群体, 每个群体包含19-130个成年个体及60-117个自然更新小苗或半同胞子代, 利用EST-SSR(simple sequence repeat based on expressed sequence tag)分子标记对各子代群体进行亲本分析, 探究鹅掌楸种间自然交配方式以检测鹅掌楸种间渐渗杂交; 同时, 以亲子群体等位基因频率的回归系数作为渐渗系数, 定量分析鹅掌楸属种间基因渐渗。结果表明: 在研究的5个鹅掌楸混生群体中, 均检测到种间渐渗杂交, 且渐渗杂交的方向与效应大小在不同群体间有差异。研究还发现, 各树种基因渐渗程度与其种群个体数有关, 种群个体数越多, 其渐渗能力越强。据此, 我们还探讨了鹅掌楸种质资源保护策略。
张红莲, 李火根 (2010) 利用EST-SSR分子标记检测鹅掌楸种间渐渗杂交. 生物多样性, 18, 120-128. DOI: 10.3724/SP.J.1003.2010.125.
Honglian Zhang, Huogen Li (2010) Detection of interspecific introgressive hybridization in Liriodendron with EST-SSR markers. Biodiversity Science, 18, 120-128. DOI: 10.3724/SP.J.1003.2010.125.
林龄 Stand age (yr) | 亲本样本 Sample size of adult trees | 子代样本 Sample size of progenies | 采样时间 Collection time | |
---|---|---|---|---|
南京群体 NJ | 31 | 130 | 自然更新小苗 Naturally regenerated seedling (96) | 2008.6 |
安吉群体 AJ-A | 24 | 19 | 自然更新小苗 Naturally regenerated seedling (60) | 2008.7 |
安吉群体 AJ-B | 24 | 43 | 自然更新小苗 Naturally regenerated seedling (80) | 2008.7 |
安吉群体 AJ-C | 18 | 38 | 自然更新小苗 Naturally regenerated seedling (80) | 2008.7 |
镇江群体 ZJ | 28 | 42 | 2个F1自由授粉子代家系 Open-polinated progeny (60、57) | 2008.6-2009.5 |
表1 各实验群体的亲、子代样本
Table 1 Sample size of adult trees and progenies from five mixture plantations with Liriodendron
林龄 Stand age (yr) | 亲本样本 Sample size of adult trees | 子代样本 Sample size of progenies | 采样时间 Collection time | |
---|---|---|---|---|
南京群体 NJ | 31 | 130 | 自然更新小苗 Naturally regenerated seedling (96) | 2008.6 |
安吉群体 AJ-A | 24 | 19 | 自然更新小苗 Naturally regenerated seedling (60) | 2008.7 |
安吉群体 AJ-B | 24 | 43 | 自然更新小苗 Naturally regenerated seedling (80) | 2008.7 |
安吉群体 AJ-C | 18 | 38 | 自然更新小苗 Naturally regenerated seedling (80) | 2008.7 |
镇江群体 ZJ | 28 | 42 | 2个F1自由授粉子代家系 Open-polinated progeny (60、57) | 2008.6-2009.5 |
自由授粉子代 Offspring | 推断父本(个体数, 比例) Deduced paternal trees (no., percentage) | 子代(个体数, 比例) Offspring (no., percentage) |
---|---|---|
F1-1 | C (2, 4.8) | F1×C (10, 16.7) |
T (5, 11.9) | F1×T (16, 26.7) | |
F1 (35, 83.3) | F1×F1 (34, 56.6) | |
F1-2 | C (2, 4.8) | F1×C (10, 17.5) |
T (5, 11.9) | F1×T (16, 28.1) | |
F1 (35, 83.3) | F1×F1 (31, 54.4) |
表2 杂交鹅掌楸自由授粉子代的父本分析及物种鉴别
Table 2 Species identification for paternities of open-pollination offspring from hybrid Liriodendron
自由授粉子代 Offspring | 推断父本(个体数, 比例) Deduced paternal trees (no., percentage) | 子代(个体数, 比例) Offspring (no., percentage) |
---|---|---|
F1-1 | C (2, 4.8) | F1×C (10, 16.7) |
T (5, 11.9) | F1×T (16, 26.7) | |
F1 (35, 83.3) | F1×F1 (34, 56.6) | |
F1-2 | C (2, 4.8) | F1×C (10, 17.5) |
T (5, 11.9) | F1×T (16, 28.1) | |
F1 (35, 83.3) | F1×F1 (31, 54.4) |
推断的亲本来源及个体数 Deduced parentage trees | 亲本组合及子代数目 Parent-pair identification and number of progenies | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
C | T | F1 | C×C | C×T or T×C | C×F1 or F1×C | T×T | T×F1 or F1×T | F1×F1 | ||
南京 NJ | 6 | 11 | 113 | 1 | 1 | 1 | 25 | 33 | 33 | |
浙江安吉 AJ-A | 14 | 3 | 2 | 50 | 4 | 3 | 2 | 0 | 1 | |
浙江安吉 AJ-B | 29 | 3 | 11 | 64 | 2 | 11 | 0 | 1 | 2 | |
浙江安吉 AJ-C | 31 | 4 | 3 | 66 | 7 | 4 | 1 | 1 | 1 |
表3 鹅掌楸混生林群体的自然交配方式
Table 3 Parent-pair identification for natural regenerated offspring in four mixture plantations of Liriodendron
推断的亲本来源及个体数 Deduced parentage trees | 亲本组合及子代数目 Parent-pair identification and number of progenies | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
C | T | F1 | C×C | C×T or T×C | C×F1 or F1×C | T×T | T×F1 or F1×T | F1×F1 | ||
南京 NJ | 6 | 11 | 113 | 1 | 1 | 1 | 25 | 33 | 33 | |
浙江安吉 AJ-A | 14 | 3 | 2 | 50 | 4 | 3 | 2 | 0 | 1 | |
浙江安吉 AJ-B | 29 | 3 | 11 | 64 | 2 | 11 | 0 | 1 | 2 | |
浙江安吉 AJ-C | 31 | 4 | 3 | 66 | 7 | 4 | 1 | 1 | 1 |
亲本群体各树种比例 (%) The percentage of three tree species | 各树种的相对繁殖贡献比例 (%) The relative reproduction contribution for three tree species | ||||||
---|---|---|---|---|---|---|---|
C | T | F1 | C | T | F1 | ||
南京 NJ | 4.6 | 8.5 | 86.9 | 2.1 | 44.7 | 53.2 | |
浙江安吉 AJ-A | 73.7 | 15.8 | 10.5 | 89.2 | 6.7 | 4.1 | |
浙江安吉 AJ-B | 67.4 | 7.0 | 25.6 | 88.1 | 1.9 | 10.0 | |
浙江安吉 AJ-C | 81.6 | 10.5 | 7.9 | 89.4 | 6.3 | 4.3 |
表4 亲本群体各树种比例及相对繁殖贡献
Table 4 The percentage of the three tree species and their relative reproduction contributions to offspring in four mixture plantations of Liriodendron
亲本群体各树种比例 (%) The percentage of three tree species | 各树种的相对繁殖贡献比例 (%) The relative reproduction contribution for three tree species | ||||||
---|---|---|---|---|---|---|---|
C | T | F1 | C | T | F1 | ||
南京 NJ | 4.6 | 8.5 | 86.9 | 2.1 | 44.7 | 53.2 | |
浙江安吉 AJ-A | 73.7 | 15.8 | 10.5 | 89.2 | 6.7 | 4.1 | |
浙江安吉 AJ-B | 67.4 | 7.0 | 25.6 | 88.1 | 1.9 | 10.0 | |
浙江安吉 AJ-C | 81.6 | 10.5 | 7.9 | 89.4 | 6.3 | 4.3 |
渐渗系数 Introgression coefficient | 南京群体 NJ | 安吉群体 AJ-A | 安吉群体 AJ-B | 安吉群体 AJ-C | 镇江群体 ZJ |
---|---|---|---|---|---|
a | 0.2549 | 0.9547 | 0.9805 | 0.9547 | 0.5658 |
b | 0.7451 | 0.0453 | 0.0195 | 0.0453 | 0.4342 |
表5 鹅掌楸与北美鹅掌楸的基因渐渗系数
Table 5 Gene introgression coefficient of Liriodendron chinense and L. tulipifera within five mixture plantations
渐渗系数 Introgression coefficient | 南京群体 NJ | 安吉群体 AJ-A | 安吉群体 AJ-B | 安吉群体 AJ-C | 镇江群体 ZJ |
---|---|---|---|---|---|
a | 0.2549 | 0.9547 | 0.9805 | 0.9547 | 0.5658 |
b | 0.7451 | 0.0453 | 0.0195 | 0.0453 | 0.4342 |
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