生物多样性 ›› 2009, Vol. 17 ›› Issue (3): 248-256.  DOI: 10.3724/SP.J.1003.2009.08316

• 论文 • 上一篇    下一篇

鸡基因组pre-microRNA SNP多态性

耿立英1, 张传生2,3,*(), 杜立新3   

  1. 1 河北科技师范学院生命科学系, 河北昌黎 066600
    2 河北科技师范学院动物科学系, 河北昌黎 066600
    3 中国农业科学院北京畜牧兽医研究所国家畜禽分子遗传育种中心, 北京 100193
  • 收稿日期:2008-12-04 接受日期:2009-02-18 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 张传生
  • 作者简介:*E-mail: cszhang1976@126.com
  • 基金资助:
    河北科技师范学院博士启动基金(2006D005);河北省自然科学基金(C2008001308)

Single nucleotide polymorphisms in chicken genomic pre-microRNA

Liying Geng1, Chuansheng Zhang2,3,*(), Lixin Du3   

  1. 1 Department of Life Science, Hebei Normal University of Science & Technology, Changli, Hebei 066600
    2 Department of Animal Science, Hebei Normal University of Science & Technology, Changli, Hebei 066600
    3 National Center for Molecular and Breeding of Animal, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193
  • Received:2008-12-04 Accepted:2009-02-18 Online:2009-05-20 Published:2009-05-20
  • Contact: Chuansheng Zhang

摘要:

为探讨鸡pre-microRNA SNP的多态性及其可能的功能意义, 对鸡471个pre-microRNA区域的SNP位点进行了鉴定和生物信息学分析。结果表明: pre-microRNA的SNP多态性显著地低于其侧翼区(P<0.01=, 其种子区SNP变异对pre-microRNA二级结构稳定性的影响高于其他各区; microRNA成熟体SNP可能影响microRNA对靶基因的选择。研究结果提示: pre-microRNA相对于其侧翼区在分子进化过程中受到更大的选择压力; 成熟体SNP可通过影响microRNA加工和靶基因的选择, 改变多种生理过程, 导致鸡种间表型变异。研究结果将为鸡microRNA的进化模式研究及其功能性SNP的鉴定提供参考信息。

关键词: 小RNA, 生物信息学, 单核苷酸多态

Abstract

The level of SNPs in chicken pre-microRNAs was studied to gain insight into the potential functional significance of the SNPs located in different domains of pre-microRNA. We conducted a bioinformatic survey of chicken SNPs in 471 pre-microRNAs, together with their flanking regions. The SNP density in pre-microRNA regions was significantly lower in the flanking regions (P<0.01=. The SNPs in the Seed regions showed greater effects on the stability of the pre-microRNA secondary structure than those SNPs in other regions did. The SNPs in the mature microRNA regions also appeared to influence target selection of the microRNA. The pre-microRNA regions may have endured more selective pressure than their flanking regions in the course of molecular evolution. It is possible that SNPs located in mature regions of microRNA are capable of modifying a number of biological processes by influencing the processing and target selection of microRNAs. This may contribute to the phenotypic variability observed among chickens. The findings reported here may provide reference information regarding the evolutionary patterns of microRNAs in chicken and may also aid in the identification of functional SNPs in microRNAs.

Key words: microRNA, bioinformatics, single nucleotide polymorphism