Biodiversity Science ›› 2000, Vol. 08 ›› Issue (4): 391-396.doi: 10.17520/biods.2000055
• 论文 •
WANG Wei-Ji, KONG Jie, ZHUANG Zhi-Meng, MENG Xian-Hong
To reveal isozyme variation of Red Seabream ( Pagrus major) , each 50 index samples of a wild
population and of a hatchery population from Haizhou Bay of Yellow Sea were investigated through starch gel
electrophoresis. Twenty loci of 13 enzymes were detected. The proportion of polymorphic loci was 45 % and
25 % ( P0.95) , respectively , ME and EST- b showed the highest genetic variation among the detected loci.
The mean heterozygosity ( Ho) in two populations were 0.141 ±0.044 and 0.095 ±0.043 , respectively. The
hatchery population possesses lower genetic variation than the wild populations. The relatively small numbers
of broodstock may result in the lower genetic variation of hatchery population. For the sustainable development
of the mariculture in the future , some appropriate measures , such as enlarging the broodstock number , should
be taken in case of the reducing of population genetic variation. The result of isozyme analyzing was compared
with that of RAPD technique in this passage , which showed that these two results were identical. So it is
meaningful to combine RAPD with isozyme technique in assessing population genetic variation.
WANG Wei-Ji, KONG Jie, ZHUANG Zhi-Meng, MENG Xian-Hong. Isozyme variation of the wild and hatchery populations of Red Seabream. (2000) Biodiv Sci, 08(4), 391-396.
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