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Diversity among the indigenous Bradyrhizobium japonicum strains from nodules on roots of the single soybean plant

Bu Changsong Zhang Xuejiang Jiang Mulan Hu Xiaojia   

  1. Oil Crops Research Institute , Chinese Agriculture Academy of Sciences , Wuhan 430062
  • Received:1995-09-01 Online:1996-11-20

Intraspecific diversity are analysized for 12 indigenous strains isolated from nodules on roots of the single soybean plant and identified as Bradyrhizobium japonicum strains. Mucoid and translucent colony type M as well as watery and transparent colony type W were observed from different colony size (diameter≥1.0 mm to ≤0.5 mm) formed by the strains on YEM agar. Serological types of the strains were identified by ELISA classfied as serocluster LL19 (including Sz1-12s serogroup Sz1-19s serotype and others) and non-serocluster LL19 (including Sz1-13s serotype Sz1-21s serotype and unknown). The chromosomal DNA's EcoRI-digested products of the strains were hybridized to the digoxigenin-Labled probe, pMJS12 plasmid, producing two kinds of single DNA hybridization fragment in the strains, sized 9.6 kb and 6.4 kb, respectively. Accordingly the strains were classfied as pattern Ⅰand pattern Ⅱ. Up to eight kinds of fatty acid in the strains were determined by HP5890 A gas-chromatography. Among them, principal component analysis of C14∶1, C18∶1and C14∶0 occured in all B. japonicum strains tested, which was greatly different from the results of fast-growing rhizobia, was used to show that the strains could be divided into six groups.

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