Biodiversity Science ›› 1996, Vol. 04 ›› Issue (2): 119-122.doi: 10.17520/biods.1996020

• 论文 • Previous Article     Next Article

DNA isolated from dried leaves of Ophiopogon xylorrhizus for Random Amplified Polymorphic DNA(RAPD) analysis

Zhang Daming, Chen Xinlu, Deng Zhengrong   

  1. 1) Laboratory of Systematic and Evolutionary Botany , the Chinese Academy of Sciences ,Beijing 100093
    2) Department of Landscape Architecture ,Beijing Agricultural College ,Beijing 102206
  • Received:1995-03-30 Revised:1995-07-17 Online:1996-05-20

Ophiopogon xylorrhizus is an endangered species,restrictedly distributed in tropical rain forest of Xishuangbanna,Yunnan,China,whihc is especially significant to studies on systematics and conservation biology of plants.RAPD is a rapid and simple method to reveal genetic diversity of populations.DNA as templets was generally isolated from fresh materials,which has difficulties in dealing with materials from remote districts.Total DNAs of 49 individuals among 4 populations were extracted using High salt low pH method from Ophiopogon xylorrhizus leaves dried through silica gel in the field.Quantity of the DNA ranged from 80 to 160 μg/dried leaves of 1 gram of fresh leaves.Compared with DNA from fresh materials,the DNA from dried leaves was not even in molecular weight and partly took place reduction,which affected PCR amplification.Good results and high production of amplification were achieved through different treatments to templet DNAs and adjustment of PCR programs.This research lays the foundation for extracting DNA from other tax using materials dried in the field for RAPD analyses.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Ruili Zhang, Yin Jia, Qixiang Zhang. Phenotypic variation of natural populations of Primula denticulata ssp. sinodenticulata[J]. Biodiv Sci, 2008, 16(4): 362 -368 .
[2] Jianyong Wu, Dayuan Xue. Important issues concerning the national legislation of access to genetic resources and benefit-sharing[J]. Biodiv Sci, 2017, 25(11): 1156 -1160 .
[3] Xingguang Dong, Yufen Cao, Kun Wang, Luming Tian, Ying Zhang, Dan Qi. Comparison of the Characters and Distribution of Vessel Elements in Xylem Among Three Main Pear Rootstocks in China[J]. Chin Bull Bot, 2015, 50(2): 227 -233 .
[4] ZHANG Yi-Shun HUANG Shang-Zhi FU Jia-Rui. Progress in Study on Raffinose Family Oligosaccharides in Seeds[J]. Chin Bull Bot, 2001, 18(01): 16 -22 .
[5] Xu Guo-wan, Zhuo Rong-zong, Cao Hao, Li Xiang-gan. Annual Changes of Biomass of Spartina alterniflora and the Relationships Between Biomass and Tidal Land Habits[J]. Chin J Plan Ecolo, 1989, 13(3): 230 -235 .
[6] Chen Cheih. On Medinilla Gaudich. of China in Relation to the Drift of the Indian Plate[J]. J Syst Evol, 1983, 21(4): 416 -422 .
[7] Jiang Gao-ming. The Impact of Globae Increasing of CO2 on Plants[J]. Chin Bull Bot, 1995, 12(04): 1 -7 .
[8] ZHOU Shuang-Xi, WU Dong-Xiu, ZHANG Lin, SHI Hui-Qiu. Effects of changing precipitation patterns on seedlings of Stipa grandis, a dominant plant of typical grassland of Inner Mongolia, China[J]. Chin J Plan Ecolo, 2010, 34(10): 1155 -1164 .
[9] YU Bing-Jun LIU You-Liang. Chlorine, Chloride Channel and Chlorine Tolerance in Plants[J]. Chin Bull Bot, 2004, 21(04): 402 -410 .
[10] Ximei Zhang, Zhichao Pu, Yuanheng Li, Xingguo Han. Stochastic processes play more important roles in driving the dynamics of rarer species[J]. J Plant Ecol, 2016, 9(3): 328 -332 .