Biodiversity Science ›› 1996, Vol. 04 ›› Issue (2): 119-122.doi: 10.17520/biods.1996020

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DNA isolated from dried leaves of Ophiopogon xylorrhizus for Random Amplified Polymorphic DNA(RAPD) analysis

Zhang Daming, Chen Xinlu, Deng Zhengrong   

  1. 1) Laboratory of Systematic and Evolutionary Botany , the Chinese Academy of Sciences ,Beijing 100093
    2) Department of Landscape Architecture ,Beijing Agricultural College ,Beijing 102206
  • Received:1995-03-30 Revised:1995-07-17 Online:1996-05-20

Ophiopogon xylorrhizus is an endangered species,restrictedly distributed in tropical rain forest of Xishuangbanna,Yunnan,China,whihc is especially significant to studies on systematics and conservation biology of plants.RAPD is a rapid and simple method to reveal genetic diversity of populations.DNA as templets was generally isolated from fresh materials,which has difficulties in dealing with materials from remote districts.Total DNAs of 49 individuals among 4 populations were extracted using High salt low pH method from Ophiopogon xylorrhizus leaves dried through silica gel in the field.Quantity of the DNA ranged from 80 to 160 μg/dried leaves of 1 gram of fresh leaves.Compared with DNA from fresh materials,the DNA from dried leaves was not even in molecular weight and partly took place reduction,which affected PCR amplification.Good results and high production of amplification were achieved through different treatments to templet DNAs and adjustment of PCR programs.This research lays the foundation for extracting DNA from other tax using materials dried in the field for RAPD analyses.

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