Biodiversity Science ›› 1995, Vol. 03 ›› Issue (2): 88-90.doi: 10.17520/biods.1995015

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Sequence analysis of a pseudogene member in the 10kDa sulfur-rich prolamin gene family of the Chineserice

Ma Jianzhong   

  1. Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:1994-05-16 Revised:1994-10-20 Online:1995-05-20

Sequence analysis of a pseudogene member in the 10kDa sulfur-rich prolamin gene family of the Chineserice/Ma Jianzhong//CHINESE BIODIVERSITY. --1995, 3(2): 88~90In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chi-nese rice. The sequence analysis showed that the amplified fragment was 380bp long, 94. 5 % homologousto the nucleic acid sequence reported by Masumura et al.[1] and 99. 5% homologous to our previous results[2]. The single base insertion between position 150 and 151 caused the reading frame mutation of thefragment and formed an amber codon at position between 162 to 164. These results showed that therewould be a pseudogene member in the 10kDa sulfur-rich prolamin gene family.

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