Biodiversity Science ›› 2007, Vol. 15 ›› Issue (4): 432-436.doi: 10.1360/biodiv.070111

• 论文 • Previous Article     Next Article

Comparison of SNP diversity between 5′-flanking regions and introns of eight functional genes in chickens

Yousheng Rao1, 2, Qinghua Nie1, Yong Liang1, Yingjun Du1, Wenlong Yan1, Xiquan Zhang1*   

  1. 1 College of Animal Science, South China Agricultural University, Guangzhou 510642
    2 Department of Biological Technology, Jiangxi Institute of Education, Nanchang 330029
  • Received:2007-04-16 Revised:2007-06-16 Online:2007-07-20

The goal of this study was to explore the level of Single Nucleotide Polymorphisms (SNP) level of 5′-flanking regions in chicken functional genes. The 5′-flanking region of eight genes, including GH, DDBC1, RIKEN, RASGRP3, IGF1, THRSP, VIP and PRL, were amplified and sequenced for White Leghorn, White Recessive Rock, Xinghua, Silkie, Gushi, and Red Jungle Fowl strains. A total length of 8,399 bp se-quence was obtained and 161 SNPs were identified, with an average of one SNP per 52 bp. Watterson’s esti-mate of θ was 0.00620 ± 0.00110 and the average nucleotide diversity (π) calculated from the average num-ber of pair-wise differences was 0.00559 ± 0.00100. The SNP diversity of 5′-flanking regions was signifi-cantly lower than that of introns. Because of their importance in regulation of gene expression, the 5′-flanking regions may have endured more selective pressure than introns during molecular evolution. A relatively low SNP diversity in 5′-flanking region may be more adoptive. The Tajima test and Fu & Li test showed that VIP and PRL, which are significantly associated with chicken productivity, are likely to be target genes for artificial and natural selection.

No related articles found!
Full text



[1] Lei Zhao, Mei Chen, Dongmei Cheng, Haomeng Yang, Yongle Sun, Heyi Zhou, and Fang Huang. Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress[J]. J Integr Plant Biol, 2013, 55(11): 1054 -1068 .
[2] ZHANG Bo, SUN Shan, ZHANG Zhi-Qiang, LI Qing-Jun. A review of the evolutionary and ecological significance of lever-like stamens[J]. Chin J Plan Ecolo, 2010, 34(1): 89 -99 .
[3] Yongmei Wu, Xue Mao, Shujian Wang, Jinai Xue, Xiaoyun Jia, Jiping Wang, Zhirong Yang, Runzhi Li. Systematic Metabolic Engineering of ω-7 Fatty Acids in Plants[J]. Chin Bull Bot, 2011, 46(5): 575 -585 .
[4] Jian-Jun Hou, Bang-Qin Huang, Zhen-Rui Cao, Ji-Xin Chen and Hua-Sheng Hong. Effects of Nutrient Limitation on Pigments in Thalassiosira weissflogii and Prorocentrum donghaiense[J]. J Integr Plant Biol, 2007, 49(5): .
[5] Niu Zi-mian Fang Yao-ren. Study on the ABSCISIC Acid in Leaf of Spur-type Variety of Apple[J]. Chin Bull Bot, 1994, 11(02): 49 -50 .
[6] Minliang Jin, Xiangguo Liu, Wei Jia, Haijun Liu, Wenqiang Li, Yong Peng, Yanfang Du, Yuebin Wang, Yuejia Yin, Xuehai Zhang, Qing Liu, Min Deng, Nan Li, Xiyan Cui, Dongyun Hao and Jianbing Yan. ZmCOL3, a CCT gene represses flowering in maize by interfering with the circadian clock and activating expression of ZmCCT[J]. J Integr Plant Biol, 2018, 60(6): 465 -480 .
[7] SONG Bao-Hua, LI Fa-Zeng. The utility of trnK intron 5′ region in phylogenetic analysis of Ulmaceae s. l.[J]. J Syst Evol, 2002, 40(2): 125 -132 .
[8] Lei Guo, Li Jiang, Xiu-Li Lu and Chun-Ming Liu. ANAPHASE PROMOTING COMPLEX/CYCLOSOME‐mediated cyclin B1 degradation is critical for cell cycle synchronization in syncytial endosperms[J]. J Integr Plant Biol, 2018, 60(6): 448 -454 .
[9] Wang Yucai, Zhang Hengjia, Deng Haoliang, Wang Shijie, Ba Yuchun. Effect of Regulated Deficit Irrigation on Water Use and Yield of Isatis indigotica[J]. Chin Bull Bot, 2018, 53(3): 322 -333 .
[10] Shuhua Guo, Yongjiang Sun, Yanjie Niu, Ning Han, Heng Zhai, Yuanpeng Du. Effect of Alkaline Salt Stress on Photosystem Activity of Grape F1 Generation Hybrids[J]. Chin Bull Bot, 2018, 53(2): 196 -202 .