Biodiversity Science ›› 2007, Vol. 15 ›› Issue (3): 282-291.doi: 10.1360/biodiv.060332

• 论文 • Previous Article     Next Article

Genetic variation and clonal diversity in fragmented populations of the desert plant Eremosparton songoricum based on ISSR markers

Xueying Lu1, 2, Daoyuan Zhang1*, Wenbao Ma3   

  1. 1 Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011
    2 Graduate University of the Chinese Academy of Sciences, Beijing 100049
    3 Xinjiang Agricultural University, Urumqi 830052
  • Online:2007-05-20

Eremosparton songoricum is a clonal shrub that reproduces both asexually by below-ground rhizomes and sexually by seeds. Having been severely disturbed by human activities, it has greatly declined in range, population number and population size over the past decades. It is now a rare species with a narrow distribution in fragmented habitat patches in the Gurbantuggut Desert of Xinjiang, China. Inter-Simple se-quence repeats (ISSR) were used to evaluate the genetic variation and clonal diversity of seven populations (148 individuals) of E. songoricum from Gurbantunggut Desert. A total of 84 discernible bands were gener-ated using eight primers of which 77 were polymorphic, indicating considerable genetic variation at the spe-cies level (PPB=91.67%, I=0.3192) and at population level (PPB =58.45%, I=0.2248) compared to other de-sert plants. A relatively high value of GST (0.2978) indicated significant genetic differentiation among populations. Analysis of molecular variance (AMOVA) revealed that 31.88% of the ISSR variation resided among populations. Barriers of gene flow caused by habitat fragmentation and population size reduction may contribute to the significant genetic differentiation among populations. Simpson diversity index (D) ranged from 0.8457–0.9400 at population level, indicating that clonal diversity was also high. The evenness of dis-tribution of genotypes in the seven populations was 0.9673 at species level, with all of the genotypes being local ones. The high level of genetic variation and clonal diversity found in E. songoricum are probably due to outcrossing or mixed-mating breeding system and multi-clone origin of populations. The results provide baseline data for formulating conservation strategies for this species. Conservation strategies should include both in situ and ex situ approaches to preserve the greatest possible extent of the genetic diversity of the species.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Hong Qian. Climatic correlates of phylogenetic relatedness of woody angiosperms in forest communities along a tropical elevational gradient in South America[J]. J Plant Ecol, 2018, 11(3): 394 -400 .
[2] WEI Cun-Xu, LAN Sheng-_Yin and XU Zhen-Xiu. Ultrastructural Features of Nucleus Degradation During Programmed Cell Death of Starchy Endosperm Cells in Rice[J]. J Integr Plant Biol, 2002, 44(12): 1396 -1402 .
[3] CHENG Han-Ting,LI Qin-Fen,LIU Jing-Kun,YAN Ting-Liang,ZHANG Qiao-Yan,WANG Jin-Chuang. Seasonal changes of photosynthetic characteristics of Alpinia oxyphylla growing under Hevea brasiliensis[J]. Chin J Plan Ecolo, 2018, 42(5): 585 -594 .
[4] Yueh Chung-Hsi, Cheng Ching-Yung. The Chinese medicinal species of the genus Trichosanthes L.[J]. J Syst Evol, 1980, 18(3): 333 -352 .
[5] Kang Gao, Juan Du, Mingyu Hou, Jing Zhao, Yanyun Pan. Research Advances in Inositol Phosphate Signaling in Regulating Pollen Development and Pollen Tube Growth[J]. Chin Bull Bot, 2013, 48(2): 210 -218 .
[6] Yu-Long Gao, Xue-Feng Yao, Wen-Zheng Li, Zhong-Bang Song, Bing-Wu Wang, Yu-Ping Wu, Jun-Li Shi, Guan-Shan Liu, Yong-Ping Li and Chun-Ming Liu. An efficient TILLING platform for cultivated tobacco[J]. J Integr Plant Biol, 0, (): 0 .
[7] WEI Yuan, WANG Shi-Jie, LIU Xiu-Ming, and HUANG Tian-Zhi. Genetic diversity of arbuscular mycorrhizal fungi in karst microhabitats of Guizhou Province, China[J]. Chin J Plan Ecolo, 2011, 35(10): 1083 -1090 .
[8] LI Yi-Ming, XU Long, MA Yong, YANG Jin-Yuan, YANG Yu-Hui. The species richness of nonvolant mammals in Shennongjia Nature Reserve, Hubei Province, China: distribution patterns along elevational gradient[J]. Biodiv Sci, 2003, 11(1): 1 -9 .
[9] Dapeng Li;Min Zhang;Qian Gao;Yong Hu;Yikun He*. An Emerging Picture of Plastid Division in Higher Plants[J]. Chin Bull Bot, 2009, 44(01): 43 -51 .
[10] MENG Ying-, YANG Yong-Ping. Chromosome Atlas of Eight Asteraceae Species from the Qinghai-Tibet Plateau[J]. Plant Diversity, 2013, 35(3): 361 -366 .