Biodiversity Science ›› 2007, Vol. 15 ›› Issue (3): 224-231.doi: 10.1360/biodiv.060263

• 论文 • Previous Article     Next Article

Species validities of Coilia brachygnathus and C. nasus taihuensis based on sequence variations of complete mtDNA control region

Wenqiao Tang, Xuelian Hu, Jinquan Yang*   

  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Fisheries University, Ministry of Education, Shanghai 200090
  • Received:2006-11-07 Revised:2007-01-21 Online:2007-05-20

We determined 32 complete mitochondrial control region (D-loop region) sequences of Coilia species, including C. nasus, C. grayii, C. mystus, C. brachygnathus and the so-called C. nasus taihuensis, to investigate the validity of disputed species C. brachygnathus and C. nasus taihuensis, and to discuss the phylogenetic relationships of Coilia in China. Engraulis japonicus and E. ringens were used as outgroups. Sequences length of C. grayii, C. mystus, C. nasus, C. brachygnathus and C. nasus taihuensis were 1,208 bp, 1,279–1,361 bp, 1,252–1,290 bp, 1,214–1,252 bp, and 1,252–1,442 bp, respectively. Except for C. grayii, other Coilia species have length variations among individuals. The average K 2-P distances among C. brachygnathus, C. nasus and C. nasus taihuensis ranged from 0.011 to 0.020, while the average distances between these three species and the species of C. grayii, C. mystus and outgroups ranged from 0.051 to 0.349, thus showing significant difference between the former and latter species. The phylogenetic trees es-timated by maximum parsimony (MP) and neighbor-joining (NJ) methods indicated that the individuals of C. nasus, C. nasus taihuensis and C. brachygnathus failed to form monophyletic clades. These results revealed that C. nasus taihuensis and C. brachygnathus should be considered the same species and C. nasus is a valid species name. The phylogenetic result also suggests that C. mystus is the most basal among the three valid species of Coilia, while C. grayii and C. mystus are more derived and are sister groups. We presume that C. mystus is the earliest descendant of Coilia ancestor which dispersed from the original center to the northwest Pacific Ocean. C. nasus and C. grayii are the species diverged from C. mystus adapted to cold and warm en-vironment, respectively.

No related articles found!
Full text



[1] GU Rui-Sheng;LIU Qun-Lu;CHEN Xue-Mei and JIANG Xiang-Ning. Comparison and Optimization of the Methods on Protein Extraction and SDS-PAGE in Woody Plants[J]. Chin Bull Bot, 1999, 16(02): 171 -177 .
[2] . [J]. Chin Bull Bot, 1994, 11(专辑): 6 .
[3] HUANG Yan ZHAO De-Xiu LI Zuo_Hu. Advances in the Study on Plant Cell Culture by Bioreactor (II)[J]. Chin Bull Bot, 2001, 18(06): 665 -671 .
[4] . [J]. Chin Bull Bot, 2000, 17(专辑): 302 -311 .
[5] Zhang Zhen and Chu Meng-yuan. Anatomic Observation on the Fruit Development of Prunus salicina[J]. Chin Bull Bot, 1991, 8(04): 52 -53 .
[6] LIU Lin FAN Shu-Guo ZHANG Zai-Jun GUO Yan-KuiZHANG Heng-YueYE Xiu-Lin LIANG Cheng-Ye. Ultrastructural Localization of Adenosine Triphosphatase Activity in Watermelon Stigma Papilla Cells During Secretion[J]. Chin Bull Bot, 2000, 17(02): 179 -184 .
[7] MA Qing-Wen GU Feng-Qi. Comparative Studies on Morphological Features of Some Genera in Taxodiaceae[J]. Chin Bull Bot, 2000, 17(专辑): 161 -164 .
[8] Yang Sheng-chang;Lin Peng and Nakasuga Tsuneo. Photosynthesis of Mangroves[J]. Chin Bull Bot, 1996, 13(增刊): 33 -38 .
[9] Lifeng Zhao;Tuanyao Chai*. Roles of AP2/EREBP Family of Transcription Factors in Development and Stress Response of Plants[J]. Chin Bull Bot, 2008, 25(01): 89 -101 .
[10] ZHANG Yi-Shun HUANG Shang-Zhi FU Jia-Rui. Progress in Study on Raffinose Family Oligosaccharides in Seeds[J]. Chin Bull Bot, 2001, 18(01): 16 -22 .