生物多样性 ›› 1998, Vol. 06 ›› Issue (4): 278-281.DOI: 10.17520/biods.1998041

• 论文 • 上一篇    下一篇

猕猴桃属(Actinidia)植物的离体种质保存

陈维伦,郭东红,安和祥,朱至清   

  1. (中国科学院植物研究所,  北京 100093)
  • 收稿日期:1997-11-12 修回日期:1998-04-01 出版日期:1998-11-20 发布日期:1998-11-20

In vitro conservation of Actinidia

CHEN Wei-Lun, GUO Dong-Hong, AN He-Xiang, ZHU Zhi-Qing   

  1. Institute of Botany , Chinese Academy of Sciences , Beijing  100093
  • Received:1997-11-12 Revised:1998-04-01 Online:1998-11-20 Published:1998-11-20

摘要:

本文研究了猕猴桃属植物离体种质资源保存的方法。在一年中于盛夏时采取的植物材料在离体培养时能得到最好的效果;改进的剥离茎尖的方法使污染率大大降低,在MS附加BA 0.5,Z 0.1,GA 0.1~0.5 mg/L,蔗糖3%,琼脂0.55%的培养基中茎尖生长良好并且不产生不定芽。通过茎尖培养方法已保存了10多个种,40多个猕猴桃的离体种质。

Abstract:

This paper deals with the methods for in vitro conservation of  Actinidia. Early summer was the best time to obtain the plant materials.The contamination rate was greatly decreased by using a improved technology to excise the shoot tips and the shoot tips grew well and had no adventitious shoot formation in MS medium supplemented with BA 0.5, Z 0.1, GA 0.1~0.5 mg/L.Up to date, about 12 species and more than 40 clones of Actinidia plants have been conserved in vitro.