生物多样性 ›› 2003, Vol. 11 ›› Issue (3): 197-203.doi: 10.17520/biods.2003026

• 论文 • 上一篇    下一篇

黄海和东海小黄鱼遗传多样性的RAPD分析

蒙子宁, 庄志猛, 金显仕, 唐启升, 苏永全   

  1. 1 (农业部海洋渔业资源可持续利用重点开放实验室,黄海水产研究所,青岛 266071)
    2 (厦门大学海洋系,厦门 361005)
  • 收稿日期:2002-12-02 修回日期:2003-01-22 出版日期:2003-05-20
  • 通讯作者: 庄志猛

Genetic diversity in small yellow croaker (Pseudosciaena polyactis) by RAPD analysis

MENG Zi-Ning, ZHUANG Zhi-Meng, JIN Xian-Shi, TANG Qi-Sheng, SU Yong-Quan   

  1. 1 Key Lab for Sustainable Utilization of Marine Fishery Resources,Ministry of Agriculture;Yellow Sea Fisheries Research Institute,Qingdao 266071
    2 Department of Oceanography,Xiamen University,Xiamen 361005
  • Received:2002-12-02 Revised:2003-01-22 Online:2003-05-20
  • Contact: ZHUANG Zhi-Meng

小黄鱼(Pseudosciaena polyactis)是我国近海重要经济鱼类之一。本文分析了采自黄海和东海5个海区共计48个个体小黄鱼的随机扩增DNA多态性(RAPD)。从40个10 bp引物中选取20个用于群体遗传多样性分析,共检测出145个位点,其中132个(91.03%) 显多态性。用Shannon多样性指数量化的平均遗传多态度为1.93(1.50~2.44),群体内和群体间的遗传变异比例分别为69%和31%;群体间的平均遗传相似度和遗传距离分别为0.9139和0.0861。用非加权配对算数平均法(UPGMA)聚类分析的结果表明,所分析的5个群体可分为3个地理群系,从分子水平上支持了过去有关学者把黄海和东海的小黄鱼划分为北、中、南3个地理群系的观点。

Pseudosciaena polyactis is a commercially important fish species which is widely distributed in the Bohai Sea, the Yellow Sea and the northern part of the East China Sea. Forty-eight individuals of P. polyactis from five sampling areas in the Yellow Sea and the East China Sea were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variation among and within the stocks. A total of 145 loci were amplified using 20 random primers, of which 132 loci (91.03%) were polymorphic. Genetic diversity quantified by Shannon index varied from 1.50 to 2.44 with an average of 1.93. Partition of genetic variation indicated that 69% was distributed within stocks and 31% among stocks. The average genetic similarity and genetic distance were 0.9139 and 0.0861, respectively. Cluster analysis by UPGMA indicated that these five stocks might be divided into three groups. Results of RAPD analysis suggested extensive genetic diversity exists in this species and the genetic divergence among stocks is relatively high. The UPGMA dendrogram showed that there existed three geographic populations of P. polyactis in the Yellow Sea and the East China Sea, which supports previous conclusions based on morphological and ecological methods.

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