生物多样性 ›› 2002, Vol. 10 ›› Issue (2): 208-212.doi: 10.17520/biods.2002027

• 论文 • 上一篇    下一篇

山东省根瘤菌表型多样性及数值分析

韦革宏, 郝鲜俊, 孙科技, 刘虎岐, 陈文新   

  1. 1 西北农林科技大学资源环境学院,陕西杨凌 712100
    2 西北农林科技大学生命科学学院,陕西杨凌 712100
    3 中国农业大学生物学院,北京 100094
  • 收稿日期:2001-07-09 修回日期:2001-12-26 出版日期:2002-05-20

Diversity of phenotypic features and numerical analysis of Rhizobia isolated from Shandong Province

WEI Ge-Hong, HAO Xian-Jun, SUN Ke-Ji, LIU Hu-Qi, CHEN Wen-Xin   

  1. 1 College of Resources and Environment,Northwest Sci-Tech Universtiy of Agriculture and Forestry,Yangling,Shanxi 712100
    2 College of Life Sciences,Northwest Sci-Tech Universtiy of Agriculture and Forestry,Yangling,Shanxi 712100
    3 College of Biological Sciences,China Agricultural University,Beijing 100094
  • Received:2001-07-09 Revised:2001-12-26 Online:2002-05-20

在山东省根瘤菌资源调查的基础上,选取31株快生根瘤菌和34株参比菌株,进行了102项表型性状测定和聚类分析。结果表明:不同地理来源、甚至同一地区来源或同种寄主的不同菌株在碳氮源利用、抗生素抗性、耐逆性等方面存在着较高的多样性。32% 的菌株可耐受300 μg/mL的新霉素,39%的菌株可耐受300 μg/mL的青霉素和洁霉素。2株菌能耐受4.0% 的NaCl。13株菌可以在pH11的条件下生长。在87%的相似水平上,未知菌株形成2个独立的亚群,第1亚群有3株菌,中心菌株为SD109;第2亚群有4株菌,中心菌株为SD083。

Based on investigation of rhizobial resources of Shandong Province, 102 phenotypic features of 31 fast growing rhizobia strains and 34 reference strains were analyzed and numerically classified. The results revealed huge diversity among strains isolated from different places. Strains with the same geographic resources and the same hosts differed in carbon and nitrogen nutrient utilization, resistance to antibiotics, and tolerance to salt, alkali and chemical dye. Thirty two percent of strains could resist 300 μg/mL Neomycin and 39 percent of strains could resist 300 μg/mL Penicillin and Jiemycin. Two strains could grow in medium with 4 % NaCl and 13 strains grew in pH 11 medium. Unknown strains were clustered into two new subgroups different from known species at the similarity level of 87%. Cluster 1 had three strains, of which SD109 was the central strain. Cluster 2 had four strains, and SD083 was the central strain.

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