生物多样性 ›› 1999, Vol. 07 ›› Issue (2): 112-118.doi: 10.17520/biods.1999016

• 论文 • 上一篇    下一篇

苜蓿、草木樨、锦鸡儿根瘤菌的表型多样性分析

阎爱民, 陈文新   

  1. (中国农业大学生物学院,  北京 100094)
  • 收稿日期:1998-03-10 修回日期:1998-12-06 出版日期:1999-05-20

Phenotypic feature diversity of Rhizobia isolated from Medicago sp., Melilotus sp. and Caragana sp.

YAN Ai-Min, CHEN Wen-Xin   

  1. China Agriculture University , Biological College , Beijing 100094
  • Received:1998-03-10 Revised:1998-12-06 Online:1999-05-20

选用48株分离自新疆和内蒙的苜蓿属、草木樨属、锦鸡儿属植物的根瘤菌菌株, 进行了营养利用、抗生素抗性、耐逆性和酶活性测定等133个表型性状分析。发现分离自同种寄主植物的根瘤菌由于地理来源的不同而存在着较大的多样性。通过数值分类,各已知种分别聚群,41株未知菌在85%的相似水平上分为3个不同于已知种的群。群1的菌株主要分自苜蓿,群2的菌株主要分自草木樨,群3的菌株分自锦鸡儿,XJ96 333(寄主为Melilotus)作为1个单株在84%的相似水平上和群1聚在一起。NM 020(寄主为Caragana)在88%的相似水平上和R.leguminosarum聚在一起; NM 183、NM 218(寄主为Caragana)在86%的相似水平上和S.fredii聚在一起; 在67%的相似水平上, XJ96 482(寄主为Medicago sativa)和其它所有供试菌株聚群。群1、2、3的所有菌株能在含2.0%(340 mM)NaCl的YMA培养基上、pH 9.0的YMA培养基、40℃的高温下生长; 群3的所有菌株还能在含3.0%(510 mM)NaCl的YMA培养基上生长;群1中90%的菌株和群2、3的所有菌株能在10℃的低温下生长。 表明群1、2、3的菌株具有较强的耐盐碱、耐高低温的特性。和已知种S.meliloti具有相同寄主的群1、群2在85%相似水平上未和S.meliloti聚群,表现了这两群菌在表型上的多样性。

133 phenotypic features (nutrition utilization , antibiotic resistance ,tolerance to NaCl and alkali , enzyme activity)of 48 strains isolated from Medicago sp. , Melilotus sp. , Caragana sp. , growing in arid regions , were analyzed. The result shows that there are great diversities among strains isolated from the the same hosts growing in different geographic origin. Through the numerical taxonomy , 41 unknown strains are clustered into three new groups , at the similarity level of 85 %. The main host plant of strains in group 1 is Medicago , the main host plant of strains in group 2 is Melilotus , the host plant of strains in group 3 is Caragana. XJ96 333 (the host plant is Melilotus) was clustered with group 1 at the similarity level of 84 %. NM 020 (the host plant is Caragana) was clustered into R. leguminosarum at the similarity level of 88 %. NM 183 and NM 218 (the host plant is Caragana) were clustered into S . fredii at the similarity level of 86 %. XJ96 482 (the host plant is Medicago sativa) was clustered with other examined strains at the similarity of 67 %. Strains in group 1 ,2 and 3 can grow on YMA with 2. 0 % (340 mM) NaCl ,pH 9. 0 and in 40 ℃. Strains in group 3 can also grow on YMA with 3. 0 % (510 mM) NaCl. 90 % of strains in group 1 and all strains in group 2 ,3 can grow at 10 ℃,which shows that all examined strains of group 1 ,2 ,3 are resistant to NaCl and alkali ,low temperature and high temperature. Group 1 and Group 2 shared a similarity of lower than 85 % with S . melioti . This shows phenotypic feature diversity of strains in the group 1 and group 2

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed