生物多样性 ›› 1999, Vol. 07 ›› Issue (2): 91-96.doi: 10.17520/biods.1999013

• 论文 • 上一篇    下一篇

湖北省三品种猪27个微卫星座位的遗传变异

樊斌, 李奎, 彭中镇, Y. Chen, C. Moran, 龚炎长, 赵书红   

  1. 1  (华中农业大学畜牧兽医学院动物分子生物学与育种实验室,  武汉 430070)
    2  (Department of Animal Science , University of Sydney , NSW 2006 , Australia )
  • 收稿日期:1998-07-17 修回日期:1998-12-29 出版日期:1999-05-20
  • 通讯作者: 李 奎1

Genetic variation of 27 microsatellite loci in three Hubei indigenous pig breeds

FAN Bin1), LI Kui1), PENG Zhong-Zhen1), Y Chen2), C Moran2), GONG Yan-Zhang1), ZHAO Shu-Hong1)   

  1. 1) Lab of Animal Molecular Biology and Breeding , College of Animal Science and Veterinary Medicine , Huazhong Agricultural University , Wuhan 430070
    2) Department of Animal Science , University of Sydney , NSW 2006 , Australia
  • Received:1998-07-17 Revised:1998-12-29 Online:1999-05-20
  • Contact: LI Kui1)

采用国际动物遗传学会(ISAG)和联合国粮农组织(FAO)共同推荐的27个微卫星标记,对湖北省3个主要地方猪种(通城猪、清平猪和阳新猪)的遗传变异进行了检测。计算出各个品种的基因杂合度、各个座位的多态信息含量及品种间的遗传距离。结果表明,3个地方猪种的平均基因杂合度分别为0.7489、0.6987和0.6273,遗传多样性比较丰富;通城猪和清平猪亲缘关系较近,而两者与阳新猪亲缘关系略远。

Genetic variability within and among three indigenous pig breeds ( Tongcheng Pig , Qingping Pig and Yangxin Pig) in Hubei Province was surveyed , by means of 27 microsatellite loci recommended by ISAG (the International Society for Animal Genetics) and FAO( Food and Agriculture Organization) . Genetic heterozygosity of each pig breed , polymorphism information content of each microsatellite locus and genetic distances among these populations were calculated. The results showed that allelic number and frequencies at the same locus obviously differed among the breeds. Some alleles were shared by all breeds , for instance 159bp , 162bp and 164bp in S0155 , and 110bp in Sw72 , while some other alleles were observed only in a single breed , such as 94bp and 96bp in Sw72 that were found solely in Yangxin Pig , 233bp , 245bp and 249bp in S0288 were in Tongcheng Pig , 249bp and 253bp in Sw90 were in Qingping Pig. Therefore , the common alleles (such as 158bp , 162bp and 164bp in S0155) are likely to be the oldest and the most distinctive for the native pig breeds of China. Geneic heterozygosity of three pig breed is 0. 7489 , 0. 6987 and 0. 6273 respectively , indication high genetic diversity. Genetic relationship between Tongcheng Pig and Qingping Pig is closer than their relationship with Yangxin Pig.

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