生物多样性 ›› 1995, Vol. 03 ›› Issue (2): 88-90.doi: 10.17520/biods.1995015

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水稻 10kDa 富硫醇溶蛋白基因家族中一个假基因拷贝的核苷酸序列分析

马建忠   

  1. (中国农业科学院生物技术研究中心,北京 100083)
  • 收稿日期:1994-05-16 修回日期:1994-10-20 出版日期:1995-05-20

Sequence analysis of a pseudogene member in the 10kDa sulfur-rich prolamin gene family of the Chineserice

Ma Jianzhong   

  1. Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:1994-05-16 Revised:1994-10-20 Online:1995-05-20

本研究以中花10号水稻为材料,利用PCR技术扩增并克隆10kDa富硫醇溶蛋白基因的成熟肽编码区。对扩增产物的核苷酸序列分析表明:本扩增产物长 380bp;与 Masumura等人[1] 发表的序列相比,有94.5%的同源性,与我们以前发表的中花10号水稻10kDa富硫醇溶蛋白基因的序列相比,有99.5%的同源性。本扩增片段第150位与151位间的单碱基(T)插入导致了该片段编码区的移码突变,并在其后的第162至164位处形成了一琥珀终止子(TAG)。这表明水稻10kDa富硫醇蛋白基因家族中确实存在不正常的假基因拷贝。

Sequence analysis of a pseudogene member in the 10kDa sulfur-rich prolamin gene family of the Chineserice/Ma Jianzhong//CHINESE BIODIVERSITY. --1995, 3(2): 88~90In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chi-nese rice. The sequence analysis showed that the amplified fragment was 380bp long, 94. 5 % homologousto the nucleic acid sequence reported by Masumura et al.[1] and 99. 5% homologous to our previous results[2]. The single base insertion between position 150 and 151 caused the reading frame mutation of thefragment and formed an amber codon at position between 162 to 164. These results showed that therewould be a pseudogene member in the 10kDa sulfur-rich prolamin gene family.

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