生物多样性 ›› 2006, Vol. 14 ›› Issue (5): 421-434.doi: 10.1360/biodiv.060056

• 论文 • 上一篇    下一篇

猕猴桃自然居群SSR遗传变异的空间自相关分析

刘亚令1, 2, 李作洲2, 张鹏飞3, 姜正旺2, 黄宏文1, 2*   

  1. 1 (华中农业大学园林学院, 武汉 430074)
    2 (中国科学院武汉植物园, 武汉 430074)
    3 (信阳农业高等专科学校园艺林学系, 河南信阳 464000)
  • 收稿日期:2006-03-20 修回日期:2006-07-15 出版日期:2006-09-20
  • 通讯作者: 黄宏文

Spatial genetic structure in natural populations of two closely related Ac-tinidia species (Actinidiaceae) as revealed by SSR analysis

Yaling Liu1, 2, Zuozhou Li2 , Pengfei Zhang3, Zhengwang Jiang2, Hongwen Huang1, 2*   

  1. 1 College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430074
    2 Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074
    3 Department of Horticulture and Forestry, Xinyang Agricaltural College, Xinyang, Henan 464000
  • Received:2006-03-20 Revised:2006-07-15 Online:2006-09-20
  • Contact: Hongwen Huang

本研究采用空间自相关分析方法对同域分布的中华猕猴桃(Actinidia chinensis)和美味猕猴桃(A. deliciosa)自然居群SSR遗传变异的空间结构进行了研究, 以探讨猕猴桃自然居群遗传变异的分布特征。选用的9对SSR引物在两物种中共扩增出104个等位基因。选择频率在20–80%的SSR等位基因, 运用等样本对频率方法分别对同域分布的中华猕猴桃和美味猕猴桃各1个居群及其中华/美味猕猴桃复合居群进行了空间自相关系数Moran’s I值计算。结果表明: 中华猕猴桃和美味猕猴桃的遗传变异在居群内均存在着一定程度的空间结构, 尽管近半数或半数以上的等位基因在居群内表现为随机分布的空间模式, 但也有相当比例(29.6–48.0%)的等位基因在种内居群中和复合居群中(河南西峡51.0 %, 陕西商南44.7 %)呈现渐变、衰退、双向衰退或侵扰模式。而且其居群内遗传变异的空间分布规律, 不论是在种内还是复合居群中都基本一致: 相距在100 m以内, 特别是30 m范围内的个体间的等位基因表现出显著性的正相关, 但随着地理距离的增大逐渐显示出负相关, 说明猕猴桃属植物的有效传粉距离可能在100 m左右, 种子散播主要集中在30 m的近距离内。猕猴桃自然居群遗传变异的空间结构是其传粉和种子散播等生物学特性与生境共同作用的结果, 其中种子近距离的散播、花粉传播的有限距离及人为干扰是最主要的因素。本研究结果揭示了这两个近缘物种居群遗传变异的空间分布特征及相互关系, 有助于进一步探讨猕猴桃属植物的遗传变异、居群扩散及其地理系统发育进化等方面的规律, 并为制定相应的保育策略和措施提供基础数据和科学依据。

关键词: 根瘤菌资源, 豆科作物, 固氮酶活性

The spatial structure of genetic variation is an important part of evolutionary and ecological genetic processes in natural populations of plants, and may provide deep insights into the conservation of species. In this paper, the spatial distribution patterns of genetic variation of two closely related sympatric species, Actinidia chinensis and A. deliciosa, were investigated using SSR markers. A total of 104 alleles was scored by nine pairs of SSR primers in two natural populations, and the alleles with frequency ranging from 20% to 80% were chosen and then used to calculate Moran’s I spatial autocorrelation coefficients for the two individual species or the species complex (A. chinensis/A. deliciosa) based on equal numbers of paired samples. Over half of the alleles were found to have a random distribution pattern within populations, while a large proportion of alleles occurred as cline, depression, double depression or intrusion patterns (29.6–48.0% within populations of each individual species, 51.0–44.7% for the two species complex, in Xixia of Henan and Shangnan of Shaanxi populations, respectively). This suggests that a moderate spatial structure of genetic variation occurred within the natural populations of the two species. Moreover, a similar spatial distribution pattern of genetic variation was found within the two populations of each individual species and in the species complex at the two sampled loca-tions. The results showed that allelic variation for individual plants within 100 m (and especially within 30 m) distance had significant positive correlation, but changed into a negative correlation with increasing distance, which may imply that the distances for pollen dispersal is about 100 m, and seed dispersal might be restricted to within 30 m. The different spatial structures of genetic variation of Actinidia natural populations were influ-enced by a combination of the biological characteristic of pollination, seed dispersal, and natural habitat. The restricted seed and pollen dispersal, and intervention of human activities were the main factors influencing the spatial pattern. The results should be of importance for further understanding of population genetic structure, population spreading and phylogeography in Actinidia, and provide baseline data for the conservation and management of these species, especially for sampling strategies for ex situ conservation.

Key words: rhizobial resource, leguminous crops, nitrogenase activity

[1] 邹长明, 王允青, 刘英, 张晓红, 唐杉. (2015) 四种豆科作物的光合生理和生长发育对弱光的响应. 植物生态学报, 39(9): 909-916.
[2] 周丽霞, 蚁伟民, 丁明懋, 陈炳辉, 曹洪麟, 易志刚. (2003) 广东省豆科植物结瘤固氮及根瘤菌资源的初步研究. 生物多样性, 11(4): 309-321.
[3] 聂湘平, 蓝崇钰, 束文圣, 张志权, 黄铭洪. (2002) 锌对大叶相思-根瘤菌共生固氮体系影响研究. 植物生态学报, 26(3): 264-268.
[4] 关秀清, 杜千有, 王继伟. (1989) 内蒙古锡林郭勒草原不同固氮植物的固氮酶活性季节变化. 植物生态学报, 13(4): 387-391.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed