生物多样性 ›› 2005, Vol. 13 ›› Issue (5): 398-406.doi: 10.1360/biodiv.050133

所属专题: 探索长江流域物种的濒危机制

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宽叶泽苔草居群内遗传多样性研究

陈进明, 王青锋*   

  1. 武汉大学生命科学学院植物系统学与进化生物学研究室, 武汉 430072
  • 收稿日期:2005-06-10 修回日期:2005-07-18 出版日期:2005-09-20
  • 通讯作者: 王青锋

Genetic diversity and structure in a natural Caldesia grandis population

Jinming Chen, Qingfeng Wang*   

  1. Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072
  • Received:2005-06-10 Revised:2005-07-18 Online:2005-09-20
  • Contact: Qingfeng Wang

采用RAPD分子标记对宽叶泽苔草(Caldesia grandis)湖南浪畔湖居群30个家系共180个子代样品进行了居群内家系间以及家系内的遗传多样性分析。从100个随机引物中筛选出12个有效引物, 共产生112条带, 其中79条表现出多态性, 占总条带数的70.5%。宽叶泽苔草各个家系多态位点百分率(PPB)在7.1%–40.2%之间。各个家系基因多样性范围在0.02–0.14之间(家系水平的基因多样性为0.10), Shannon指数的范围在0.04–0.21之间(家系水平的Shannon指数为0.18)。对宽叶泽苔草30个家系的AMOVA分析结果显示, 家系间的基因分化系数Gst = 0.231, 即总的变异中有23.1%的变异存在于家系间, 家系内的遗传变异占总遗传变异的76.9%, 家系间和家系内变异均极显著(P<0.001)。采用UPGMA法对宽叶泽苔草30个家系180个子代进行聚类的结果表明: 同一家系的子代个体并不能完全聚在一起。基于家系间的遗传分化系数Gst对宽叶泽苔草30个家系间的基因流进行估测, 结果显示: Nm = 0.83, 表明宽叶泽苔草各个家系之间有较高的基因流。

Genetic diversity in a natural Caldesia grandis population in Hunan Province was assessed using RAPD markers. A total of 180 individuals from 30 maternal plants were assayed. Of the 100 RAPD primers screened, twelve produced highly reproducible bands. Using these primers, a total of 112 DNA fragments were generated with 79 (70.5%) being polymorphic. Analysis of molecular variance (AMOVA) show that a large proportion of genetic variation (76.9%) resided within families, while only a small proportion (23.1%) resided among families. Shannon index showed that total genetic diversity of maternal plants was 0.18, which was identical with the result of analysis of Nei’s gene diversity. NTSYS analysis indicated that the individual offspring from a single maternal plant could not be clustered together completely. The estimate of gene flow (Nm) among maternal plants was 0.83, indicating high gene flow among the maternal plants.

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