生物多样性 ›› 2005, Vol. 13 ›› Issue (1): 20-26.doi: 10.1360/biodiv.040127

• 论文 • 上一篇    下一篇

海南五指山猪遗传多样性的微卫星分析

王希龙1, 3, 欧江涛1*, 黄礼光1*, 郭春华2, 钟金城2, 李小成2, 王峰1, 郑心力1   

  1. 1 (海南农业科学院畜牧兽医研究所,海口571100)
    2 (西南民族大学生命科学与技术学院,成都610041)
    3 (中国农业大学动物科技学院,北京100094)
  • 收稿日期:2004-09-06 修回日期:2004-12-11 出版日期:2005-01-20
  • 通讯作者: 欧江涛

Genetic diversity in the Wuzhishan pig from Hainan based on 32 microsatellite loci

Xilong Wang1, 3, Jiangtao Ou1*,  Liguang Huang1*,  Chunhua Guo2, Jincheng Zhong2, Xiaocheng Li2,  Feng Wang1,  Xinli Zheng1   

  1. 1 Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100
    2 College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041
    3 College of Animal Science and Technology, China Agricultural University, Beijing 100094
  • Received:2004-09-06 Revised:2004-12-11 Online:2005-01-20
  • Contact: Jiangtao Ou

海南五指山猪(Wuzhishan pig)是我国珍稀的小型猪品种。它的解剖学、生理学、疾病发生机理等都与人类极其相似,具有重要的应用价值,可用于心血管病、皮肤烧伤和新药评价等许多方面的研究。作者利用多重PCR和基因扫描技术对五指山猪32个微卫星基因座进行了遗传检测。统计了全群、核心群和近交群的等位基因组成,并计算了它们的平均纯合率、多态信息含量(PIC)和平均杂合度。结果显示,全群、核心群和近交群32个基因座的平均等位基因数分别为14.590个、9.410个、13.660个,平均基因纯合率分别为44%、44.3%、44%,平均PIC分别为0.742、0.708、0.731,平均杂合度分别为0.560、0.558、0.559。这表明海南五指山猪虽然遗传基础比较稳定,但仍具有较大的遗传变异。上述结果对于海南五指山猪的保种、定向选育、开发利用都具有重要的指导意义。

Wuzhishan pig, which originated from Hainan Province, China, is one of China′s rare miniature pig breeds. It is a potential genetic resource for sustainable pig production. In addition, it can be utilized as an experimental animal for medical and veterinary research due to its small size with the mature body weight of only about 25 kg. We detected its genetic polymorphisms using 32 microsatellites distributed on 14 chromosomes. A total of 200 individuals were sampled from the nuclear herd and from an inbred herd at the Wuzhishan Pig Breeding Farm in Hainan Province and analysed by multiplex PCR and gene scanning techniques. The average numbers of alleles at the 32 marker loci were 9.410, 13.660, and 14.590 in the nuclear group, the inbred group, and the merged group, respectively. The average homozygosity was almost the same in the three groups, about 44% in each case. The polymorphism information content in the three groups was 0.708, 0.731, and 0.742, respectively. The results indicate a rich genetic polymorphism in Wuzhishan pig.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed