纳木措可培养丝状真菌多样性及其与理化因子关系
- 西藏大学理学院, 拉萨 850000
收稿日期: 2021-11-23
录用日期: 2022-04-03
网络出版日期: 2022-05-18
基金资助
国家自然科学基金(31960024);2019年中央支持地方高校改革发展基金(藏财预指[2019] 01号);1号-20号中央支持地方高校专项高层次人材引进经费(藏财预指[2020])
Relationship between culturable filamentous fungal diversity and environmental factors in Nam Co Lake
- School of Sciences, Tibet University, Lhasa 850000
Received date: 2021-11-23
Accepted date: 2022-04-03
Online published: 2022-05-18
摘要
微生物多样性在评估水体生态环境方面发挥着重要作用。本研究以青藏高原纳木措湖为研究对象, 开展水体可培养丝状真菌多样性及影响因子研究。通过膜过滤平置培养法、经典分类法和rRNA转录间隔区(ITS)序列分析对纳木措湖20个采样点的丝状真菌进行分离、纯化及鉴定, 测定水体理化指标, 综合分析丝状真菌空间分布格局与理化因子的相关性。菌种鉴定结果显示, 从纳木措水体样品中共分离纯化出1,412株丝状真菌, 隶属22属47种, 其中链格孢属(Alternaria)、青霉属(Penicillium)和毛霉属(Mucor)为优势属, 链格孢(Alternaria chlamydosporigena)和冻土毛霉(Mucor hiemalis)为优势种; Pearson相关性分析显示, 丝状真菌总丰度与温度、铵态氮、全磷呈显著正相关; 冗余分析显示, 铵态氮、温度、全磷、全氮、盐度及电导率是影响纳木措湖丝状真菌群落组成与分布的主要理化因子。综上所述, 纳木措水体可培养丝状真菌具有较高的物种多样性和空间异质性, 而且水体环境因子影响其分布。
本文引用格式
薛文凯, 孟华旦尚, 王艳红, 朱攀, 德吉, 郭小芳 . 纳木措可培养丝状真菌多样性及其与理化因子关系[J]. 生物多样性, 2022 , 30(6) : 21473 . DOI: 10.17520/biods.2021473
Abstract
Aims: Microbial diversity plays an important role in assessing the ecological environment of water bodies. This study aims to investigate the diversity and influencing factors of culturable filamentous fungi in Nam Co Lake, Qinghai-Tibet Plateau.
Methods: Filamentous fungi from 20 sites in Nam Co Lake were isolated, purified and identified by membrane filtration flat culture, morphological characterization and ITS sequence analysis. Water physical and chemical variables were determined to analyze the correlation between filamentous fungal diversity and environmental factors.
Results: Species identification results showed that a total of 1,412 filamentous fungal strains were isolated from Nam Co Lake water samples, and were identified as 47 species in 22 genera. Alternaria, Penicillium and Mucor are the dominant genera, Alternaria chlamydosporigena and Mucor hiemalis are the dominant species. Pearson correlation analysis showed that the total abundance of filamentous fungi was significantly positively correlated with temperature, ammonium nitrogen and total phosphorus. Redundancy analysis showed that ammonium nitrogen, temperature, total phosphorus, total nitrogen, salinity and electrical conductivity are important factors influencing the composition and distribution of filamentous fungal communities in Nam Co Lake.
Conclusion: In summary, the filamentous fungal community from Nam Co Lake showed high species diversity and spatial heterogeneity, and the distribution of filamentous fungi was affected by environmental factors in Nam Co Lake.
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