生物多样性 ›› 2004, Vol. 12 ›› Issue (2): 269-273.doi: 10.17520/biods.2004032

• 论文 • 上一篇    下一篇

濒危植物元宝山冷杉的遗传多样性研究

王燕1, 唐绍清1*, 李先琨2   

  1. 1 (广西师范大学生物系,桂林 541004)
    2 (广西壮族自治区中国科学院广西植物研究所,桂林 541006)
  • 收稿日期:2003-06-12 修回日期:2003-09-10 出版日期:2004-03-20
  • 通讯作者: 唐绍清

The genetic diversity of the endangered plant Abies yuanbaoshanensis

WANG Yan1, TANG Shao-Qing1*, LI Xian-Kun2   

  1. 1 Department of Biology,Guangxi Normal University,Guilin 541004
    2 Guangxi Institute of Botany,Guangxi Zhuang Autonmous Region and Chinese Academy of Sciences,Guilin 541006
  • Received:2003-06-12 Revised:2003-09-10 Online:2004-03-20
  • Contact: TANG Shao-Qing

元宝山冷杉(Abies yuanbaoshanensis)是仅分布于广西融水县元宝山的珍稀濒危物种。本研究采用AFLP分子标记分析了元宝山冷杉种群的遗传多样性。取元宝山冷杉种群43棵植株作为研究材料, 4对引物组合用于AFLP扩增,共得到261条DNA扩增带。分析结果:元宝山冷杉种群的多态位点百分比率为50.96%,Nei′s 基因多样性为0.1510, Shannon 多样性指数为0.1735。用NTSYS软件计算各个样品间的相似性系数,并用UPGMA法基于相似性系数进行了聚类分析。研究结果表明: 元宝山冷杉种群的遗传多样性水平低,种群内个体间的相似性系数很大,无明显亚种群间的遗传分化。要保护或保存这个物种的遗传资源,建议应该选择种群内尽可能多的个体进行保护。

Abies yuanbaoshanensis (Pinaceae) is an endangered species endemic to Yuanbaoshan Mountain, Guangxi, China. Genetic diversity in 43 individuals of the speices was assessed using the amplified fragment length polymorphisms (AFLP) technique. Four primer combinations generated a total of 261 bands, of which 133 were polymorphic (50.96%). The Nei′s gene diversity of the population was 0.1510, and Shannon′s information index was 0.1735. The results indicate that Abies yuanbaoshanensis has a low level of genetic diversity. This finding provides valuable information for formulating future conservation programs for the species.

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