生物多样性 ›› 2003, Vol. 11 ›› Issue (2): 118-124.DOI: 10.17520/biods.2003016

• 论文 • 上一篇    下一篇

5个泥蚶群体遗传多样性的RAPD分析

李太武1,李成华1,2,3,宋林生2,苏秀榕1   

  1. 1 (宁波大学生命科学与生物工程学院,宁波 315211)
    2 (中国科学院海洋研究所实验海洋生物学开放实验室,青岛 266071)
    3 (中国科学院研究生院,北京 100039)
  • 收稿日期:2002-09-30 修回日期:2003-01-07 出版日期:2003-03-20 发布日期:2003-03-20
  • 通讯作者: 李太武

RAPD variation within and among five populations of Tegillarca granosa

LI Tai-Wu1, LI Cheng-Hua1,2,3, SONG Lin-Sheng2, SU Xiu-Rong1   

  1. 1 Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211
    2 Experimental Marine Biology Laboratory,Institute of oceanology,Chinese Academy of Sciences,Qingdao 266071
    3 Graduate student Faculty,Chinese Academy of Sciences,Beijing 100039
  • Received:2002-09-30 Revised:2003-01-07 Online:2003-03-20 Published:2003-03-20
  • Contact: LI Tai-Wu

摘要: 采用随机扩增多态性DNA(RAPD)技术,对5个泥蚶群体的遗传多样性进行了研究。在选择的20个随机引物中共检测到154个扩增片段,长度在300 bp~2300 bp之间,每个个体扩增条带在1~11条不等。利用Popgen 3.2和PHILIP统计软件,获得实验数据,确立了5个群体间的亲缘关系。结果表明:(1)泥蚶的5个地理群体分化不明显,没有形成不同的地理种群;(2)聚类分析显示,韩国群体与浙江群体亲缘关系最近,然后依次为山东群体和福建群体;(3)5个群体无论是在多态位点比例还是在平均遗传杂合度上都处于较高水平,说明泥蚶当前种质资源状况良好,遗传多样性水平较高,泥蚶养殖有很好的发展前景;(4)与韩国野生群体相比,其他4个泥蚶群体在多态位点比例和遗传平均杂合度上都有不同程度的降低,表明人为等因素已经开始影响到泥蚶的种质资源状况。因此,为持续发展泥蚶养殖业,必须加强保护泥蚶现有的种质资源并制定相应的渔业管理措施。

Abstract: The Randomly Amplified Polymorphic DNA(RAPD)technique was applied to assess the genetic variation among five populations of Tegillarca granosa. Under predetermined optimal reaction conditions, 154 RAPD sites were detected ranging from 300 bp to 2300 bp with 20 primers. The number of amplified bands ranged from 1 to 11 in every individual. The experimental data were analyzed with the Popgen 3.2 and PHILIP software. The results showed that: (1)no evident differentiation existed among the five populations, and they all belong to one community; (2) the nearest phylogenetic relationship occurs between the Korean population and Zhejiang populations, then between the Shandong and Fujian groups in turn; (3) the high level of mean heterozygosities suggests that the resource of Tegillarca granosa was in good condition with high genetic variation, and (4)the heterozygosities and proportion of polymorphic loci of the hatchery populations were lower than that of the wild population, which could be attributed to artificial or other factors. We suggest that it is important to take measures soon to maintain the germ plasm resource of Tegillarca granosa, so as to promote sustainable development of its aquaculture.