生物多样性 ›› 1998, Vol. 06 ›› Issue (2): 99-104.doi: 10.17520/biods.1998015

• 论文 • 上一篇    下一篇

我国部分白菜型油菜 RAPD 的研究

朱莉, 李汝刚, 伍晓明, 伍宁丰, 范云六, 钱秀珍   

  1. 1 (中国农业科学院生物技术研究中心,  北京 100081)
    2 (中国农业科学院油料作物研究所,  武汉 430062)
  • 收稿日期:1996-07-09 修回日期:1997-08-04 出版日期:1998-05-20

Genetic diversity among Chinese oilseed accessions of Brassica rapa as determined by RAPD markers

ZHU Li1), LI Ru-Gang1), WU Xiao-Ming2), WU Ning-Feng1), FAN Yun-Liu1), QIAN Xiu-Zhen2)   

  1. 1) Biotechnology Research Center , Chinese Academy of Agricultural Sciences , Beijing  100081
    2) Institute of Oil Crop , Chinese Academy of Agricultural Sciences , Wuhan  430062
  • Received:1996-07-09 Revised:1997-08-04 Online:1998-05-20

本研究用RAPD技术和统计学方法,对以湖南和湖北省油菜为主的34个白菜型油菜品种的遗传多样性进行了分析。根据离差平方和,用Ward's聚类方法进行聚类。结果表明:白菜型油菜的遗传变异与生态地理分布有密切的关系;湖南和湖北两省的白菜型油菜品种存在广泛的遗传变异;在DNA水平上可将所分析的34个品种分成8个类群。作者对研究结果在遗传育种中的应用进行了讨论。

The genetic diversity among 34 Chinese oilseed accessions ( mainly from Hunan and Hubei Provinces) of B . rapa was analyzed by RAPD technique and statistical methods. A general molecular method for assessment of genetic diversity was established from this research. Cluster analysis showed that the genetic variation among oilseed accessions of B . rapa was closely related with their eco-geographic distribution ; extensive variation existed among the accessions from Hunan and Hubei Provinces ; 34 accessions were divided into eight groups at DNA level. The application of results to selection of parents in breeding program was discussed.

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