生物多样性 ›› 2006, Vol. 14 ›› Issue (4): 309-314.doi: 10.1360/biodiv.060025

• 论文 • 上一篇    下一篇

Bt/CpTI转基因稻及其非转基因亲本对照在间隔种植条件下的转基因漂移

戎俊1, 宋志平1, 苏军2, 夏辉1, 王锋2, 卢宝荣1*   

  1. 1 (复旦大学生物多样性科学研究所, 生物多样性与生态工程教育部重点实验室, 上海 200433)
    2 (福建省农业科学院农业遗传工程重点实验室, 福州 350003)
  • 收稿日期:2006-01-23 修回日期:2006-05-10 出版日期:2006-07-20
  • 通讯作者: 卢宝荣

Low frequencies of transgene flow between Bt/CpTI rice and their non-transgenic counterparts under alternating cultivation

Jun Rong1, Zhiping Song1, Jun Su2, Hui Xia1, Feng Wang2, Bao-Rong Lu1*   

  1. 1 The Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiver-sity Science, Fudan University, Shanghai 200433
    2 Fujian Province Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou 350003
  • Received:2006-01-23 Revised:2006-05-10 Online:2006-07-20
  • Contact: Bao-Rong Lu

随着转基因技术的迅速发展, 越来越多的转基因作物被培育出来。转基因作物的外源转基因通过花粉传播向非转基因作物的漂移, 会影响非转基因作物品种的种子纯度, 从而可能导致一系列生物安全问题。为了研究转基因栽培水稻(Oryza sativa)中的外源转基因通过花粉介导向非转基因水稻品种逃逸的可能性及其频率, 我们选用3个含双价抗虫基因(Bt/CpTI)的转基因水稻品系及其相对应的非转基因水稻亲本品种(近等基因系)进行了转基因漂移的实验。为了获取在近距离状况下转基因水稻与非转基因水稻品种之间的转基因漂移频率, 采用了转基因与非转基因水稻品种间隔种植的栽培方式, 分别在福建省福州市和海南省三亚市的转基因环境安全实验地进行实验, 并利用潮霉素抗性筛选标记基因来鉴定转基因和非转基因稻的杂种。共检测了从非转基因水稻品种随机收获的70,056颗种子, 以此计算转基因漂移频率。结果表明, 在相邻种植的情况下, 由这3个转基因水稻向对应的非转基因水稻品种的转基因漂移的频率比较低(0.275–0.832%)。如此近距离条件下获得的低转基因漂移频率表明, 对于严格自花授粉的水稻而言, 通过一定的隔离措施, 能有效地降低由花粉介导的转基因漂移导致的非转基因种子混杂。

With the fast development of biotechnology, many transgenic crops have been developed and are in the pipelines for environmental release or commercialization. Crop-to-crop transgene flow from transgenic to non-transgenic rice varieties will affect seed purity of non-transgenic rice varieties, which might lead to unwanted biosafety problems. To assess the probability of transgene outflow in rice (Oryza sativa), gene flow experiments were conducted at two confined biosafety experimental sites in Fuzhou of Fujian Province and Sanya of Hainan Province, with the field design of alternating rows of transgenic rice lines and non-transgenic varieties. Three transgenic rice lines containing two insect-resistance genes (Bt/CpTI) and their non-transgenic counterparts (isogenic lines) were used in the experiments to achieve high levels of spontaneous outcrossing. A hygromycin resistance gene tightly linked with the target transgenes was used as a selective marker for identifying transgenic hybrids. Based on examination of 70,056 geminated seeds, we observed low frequencies of transgene outflow (0.275–0.832%) under cultivation of alternating rows between transgenic rice and its non-transgenic counterparts. This result of low crop-to-crop transgene flow in rice at such close spacing can provide scientific support for the safe use of transgenic rice in terms of “contamina-tion” of non-transgenic rice caused by pollen-mediated transgene outflow, if certain isolation measures are taken.

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