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生物多样性 >

2022 , Vol. 30 >Issue 7: 21488

DOI: https://doi.org/10.17520/biods.2021488

研究报告: 动物多样性

不同干旱水平下蚯蚓对番茄抗旱能力的影响

  • 李季蔓 ,
  • 靳楠 ,
  • 胥毛刚 ,
  • 霍举颂 ,
  • 陈小云 ,
  • 胡锋 ,
  • 刘满强
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  • 1.南京农业大学资源与环境科学学院土壤生态实验室, 南京 210095
    2.江苏省有机固体废弃物资源化协同创新中心, 南京 210095
*E-mail: liumq@njau.edu.cn

收稿日期: 2021-11-27

  录用日期: 2022-01-08

  网络出版日期: 2022-01-09

基金资助

国家自然科学基金(42177286);国家自然科学基金(41771287);中央高校基本科研业务费专项资金

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Effects of earthworm on tomato resistance under different drought levels

  • Jiman Li ,
  • Nan Jin ,
  • Maogang Xu ,
  • Jusong Huo ,
  • Xiaoyun Chen ,
  • Feng Hu ,
  • Manqiang Liu
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  • 1. Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095
    2. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095

Received date: 2021-11-27

  Accepted date: 2022-01-08

  Online published: 2022-01-09

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摘要

土壤无脊椎动物可能会通过促进土壤持水能力和增加土壤肥力而缓解植物的干旱胁迫。本研究采用蚯蚓和干旱水平的双因子完全交互设计, 模拟了干旱胁迫条件下蚯蚓对土壤性质及番茄抗旱性的影响。结果表明, 在高干旱胁迫时, 蚯蚓通过增加番茄茎叶抗氧化能力提高了植物抗旱性, 上调番茄茎叶脱落酸和茉莉酸生物合成过程的基因表达(NCED、NSY、OPR、AOS和LOX), 促进脱落酸和茉莉酸含量分别增加43.2%和33.6%, 过氧化氢酶、过氧化物酶和超氧化物歧化酶含量分别增加12.9%、8.4%和47.3%。在低干旱胁迫时, 蚯蚓上调茉莉酸合成通路基因表达, 但降低了脱落酸含量, 对转录因子ABF4、MYC2基因表达和植物抗氧化能力无明显影响。干旱导致的土壤水分和养分条件变化影响着蚯蚓介导的植物抗旱性响应。本研究证明了土壤动物对植物抗旱的重要作用, 如蚯蚓对植物激素合成、信号传导和抗氧化能力的影响。了解土壤动物影响植物抗旱的内在机制, 有助于深挖和利用土壤动物的多样化生态功能。

关键词： 蚯蚓; 气候变化; 地上和地下部; 植物激素; 信号通路

本文引用格式

李季蔓 , 靳楠 , 胥毛刚 , 霍举颂 , 陈小云 , 胡锋 , 刘满强 . 不同干旱水平下蚯蚓对番茄抗旱能力的影响[J]. 生物多样性, 2022 , 30(7) : 21488 . DOI: 10.17520/biods.2021488

Abstract

Aims: Soil invertebrates have the potential to modify plant responses to drought stress. For example, some functional groups improve soil conditions via resource provision and water retention, potentially alleviating drought stress. This study was designed in order to mechanistically understand the functional roles of soil fauna in ecosystem services.
Methods: A pot experiment using tomatoes (Lycopersicon esculentum) was conducted in a greenhouse. This study adopted a complete factorial design that manipulated earthworm (Metaphire guillelmi) abundance and drought stress level.
Results: Our results indicate that earthworms promote plant drought resistance under high drought stress, which significantly up-regulated gene expression (NCED, NSY, OPR, AOS and LOX) of abscisic acid and jasmonic acid biosynthesis, promoted the accumulation of abscisic acid and jasmonic acid by 43.2% and 33.6%, up-regulated transcription factor expression, and increased the activities of catalase, peroxidase and superoxide dismutase by 12.9%, 8.4% and 47.3% in tomato shoots, respectively. However, under low drought stress, earthworms up-regulated jasmonic acid synthesis pathway gene expression, but reduced abscisic acid content, and had no significant effect on the expression of transcription factors ABF4 and MYC2 genes or plant antioxidant capacity.
Conclusion: The effects of earthworms on plant resistance depends on drought level. This study confirms the importance of soil invertebrates in plant drought resistance including the potential impact of earthworms on plant hormone synthesis, signal transduction, and antioxidant capacity.

Key words： earthworms; global climate change; aboveground-belowground; plant hormone; signal transduction

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