基于环境DNA宏条形码技术研究黄河三角洲典型潮沟系统鱼类多样性及其对水文连通性的响应

董志远, 陈琳琳, 张乃鹏, 陈莉, 孙德斌, 倪艳梅, 李宝泉

Response of fish diversity to hydrological connectivity of typical tidal creek system in the Yellow River Delta based on environmental DNA metabarcoding

Zhiyuan Dong, Linlin Chen, Naipeng Zhang, Li Chen, Debin Sun, Yanmei Ni, Baoquan Li

表1 黄河口潮沟环境理化指标。同行不同小写字母表示差异显著(P < 0.05)。加粗表示最大值。

Table 1 Physical and chemical indicators of in the tidal creek of the Yellow River Estuary. Different lowercase letters in the same row meant significant difference at 0.05 level. Bolded numbers indicate maximum values. TN, Total nitrogen; TP: Total phosphorus; T: Temperature; DO: Dissolved oxygen; SPC: Conductivity; SAL: Salinity; Eh: Oxidation-reduction potential.

变量 Variable	潮沟级别 Tidal creek level
变量 Variable	一级潮沟 The first-class tidal creek (S1)	二级潮沟 The second-class tidal creek (S2)	三级潮沟 The third-class tidal creek (S3)
总氮 TN (μg/L)	351.893 ± 4.857^b	361.896 ± 11.934^ab	389.191 ± 19.168^a
总磷 TP (μg/L)	15.116 ± 1.175^a	12.586 ± 0.580^b	14.975 ± 0.715^a
磷酸盐 PO₄^3--P (mg/L)	0.033 ± 0.029^a	0.008 ± 0.002^ab	0.004 ± 0.001^b
铵盐 NH₄⁺-N (mg/L)	0.039 ± 0.012^a	0.036 ± 0.004^a	0.170 ± 0.121^b
亚硝酸盐 NO₂^--N (mg/L)	0.019 ± 0.003^b	0.024 ± 0.004^ab	0.027 ± 0.004^a
硅酸盐 SiO₃^2--Si (mg/L)	0.675 ± 0.052^b	0.690 ± 0.107^ab	0.964 ± 0.222^a
硝酸盐 NO₃^--N (mg/L)	0.265 ± 0.041^a	0.238 ± 0.018^a	0.193 ± 0.017^b
温度 T (℃)	21.800 ± 1.595^b	21.450 ± 0.321^b	26.233 ± 2.850^a
溶解氧 DO (mg/L)	7.998 ± 0.804^a	6.692 ± 0.315^b	6.067 ± 1.143^b
电导率 SPC (ms/cm)	26.412 ± 0.245^b	32.538 ± 1.752^ab	36.300 ± 1.715^a
盐度 SAL	16.185 ± 0.170^b	20.362 ± 1.202^ab	22.903 ± 1.121^a
酸碱度 pH	8.408 ± 0.012^a	8.385 ± 0.021^ab	8.248 ± 0.026^b
氧化还原电位 Eh	-71.950 ± 1.104^a	-70.583 ± 1.087^a	-64.033 ± 1.219^b