基于DNA条形码技术构建王朗国家级自然保护区陆生脊椎动物遗传资源数据库及物种鉴定

邢超, 林依, 周智强, 赵联军, 蒋仕伟, 林蓁蓁, 徐基良, 詹祥江

The establishment of terrestrial vertebrate genetic resource bank and species identification based on DNA barcoding in Wanglang National Nature Reserve

Chao Xing, Yi Lin, Zhiqiang Zhou, Lianjun Zhao, Shiwei Jiang, Zhenzhen Lin, Jiliang Xu, Xiangjiang Zhan

表3 样品经引物扩增的成功率、识别率

Table 3 The success rate and efficiency of samples amplified by PCR primers

物种类型(样品数量/个) Category (No. of samples) 成功扩增样品数(扩增成功率) No. of successfully amplified samples (Success rate) 通用引物鉴定物种数(识别率) No. of species identified by universal primers (Identification rate) 特异性引物鉴定物种数(识别率) No. of species identified by specific primers (Identification rate) 鸟类 Avians (156) 128 (82.1%) 41 (89.1%) 5 (10.9%) 兽类 Mammals (137) 111 (81.0%) 15 (55.6%) 12 (44.4%) 两栖类 Amphibians (21) 21 (100%) 3 (75.0%) -