土壤动物的分子分类预测策略评估

徐聪, 张飞宇, 俞道远, 孙新, 张峰

Performance evaluation of molecular taxonomy assignment tools for soil invertebrates

Cong Xu, Feiyu Zhang, Daoyuan Yu, Xin Sun, Feng Zhang

表2 5款分类预测软件的推荐使用情况

Table 2 The recommendation on application of five taxonomic assignment tools

分类预测软件 Tools 推荐使用情况 Recommendation on application EPA-NG 以COI作为分子标记且参考数据库不大的场合 COI is used as the marker and the reference database is small VSEARCH 以16S或18S作为分子标记或者参考数据库较大的场合 16S/18S is used as the marker; the reference database includes thousands of sequences or more HS-BLASTN 同VSEARCH, 但优先级不如VSEARCH Similar to VSEARCH APPLES 仅预测较高阶元的场合 Predicting higher taxonomical hierarchy RAPPAS 目标序列间长度差异较大的场合 When sequence lengths differ greatly