目标序列长度
Targeted region length (bp) | 优势
Advantages | 劣势
Disadvantages | 参考文献
Reference | | ~300 | - | 无法处理较长的目标序列; Roche 454平台
Can not work on long fragments;
Roche 454 platform | Shokralla et al, 2014 | | ~180 | 简单, 易操作, 成本低
Straightforward, easy to operate,
cost-efficient | 目标序列偏短, 只能用于物种初筛
Short targeted region; can only be used
for species pre-clustering | Meier et al, 2016 | | ~650 | 标准DNA条形码全长
Standard full-length COI | 普适性差; 需要多轮PCR过程
Poor universality; multiple rounds of PCR | Shokralla et al, 2015;
Cruaud et al, 2017 | | ~650 | 易操作, 标准DNA条形码全长
Easy to operate, standard full-length COI | 相对较高的计算资源
Relatively high requirement for
computational resources | Liu et al, 2017 | | ~650 | 易操作, 标准DNA条形码全长
Easy to operate, standard full-length COI | SMRT平台成本高
High cost of SMRT platform | Hebert et al, 2018 | | ~650 | 易操作, 标准DNA条形码全长
Easy to operate, standard full-length COI | 测序平台暂时不够普及
Not a mass production | Yang et al, 2018 |
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