野生和养殖大鲵群体遗传多样性的微卫星分析
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孟彦, 杨焱清, 张燕, 肖汉兵
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A comparison of genetic diversity between wild and cultured populations of the Chinese giant salamander, Andrias davidianus, based on microsatellite analyses
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Yan Meng, Yanqing Yang, Yan Zhang, Hanbing Xiao
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表1 10个大鲵微卫星座位的信息
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Table 1 Information of ten microsatellite loci in Andrias davidianus
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座位
Locus | 引物序列
Primer sequence (5'-3') | 片段大小
Size (bp) | 重复类型
SSR motif | 退火温度
Annealing
temperature (℃) | GS82
| F: CGTAGGGGTTACAAAATCA | 227 | (AG)20 | 48.0 | | R: CAGTCTGTGCCATTCATTTC | | GS17 | F: TTCTCATTTTGTTTCCTATTGC | 266 | (CT)20 | 48.0 | | R: CACAGTAAGGACGCAGTAAAA | | GS120 | F: TCTCATTTTGTTTCCTATTG | 239 | (CT)20 | 54.5 | | R: GTTGGAAATTAAAGGCAC | | GS134 | F: TTCTCACCAATATGCCACC | 168 | (GA)17 | 60.8 | | R: TCAACTTTGCTGAAAATCCA | | GS66 | F: GTGATTACAACTCGTGGACTG | 205 | (GTGA)20 | 60.8 | | R: GTAGTCTTGGTGGGAGGGTAG | | GS132 | F: CATACATCTACAACTACATCCGA | 206 | (AC)30 | 59.0 | | R: TCTTCAAGCGAGCTTTTACT | | GS96 | F: ACAGTAAGGACGCAGTAAA | 163 | (AG)20 | 60.0 | | R: TCTAACCTACCACTCCTGCT | | GS78 | F: ATTGGGGAGAAATAAAGTG | 219 | (TAGA)20 | 53.0 | | R: GTGCTTGCACAACCTAATC | | GS105 | F: CTTACATTTCGCACGGATTT | 260 | (AG)5…(AG)6 | 56.5 | | R: ATGGCACATTGTAGGAGTTT | | GS122 | F: TTCAGGAGGGACAGGGAGA | 370 | (AG)10…(AG)5…(AG)5…(AG)5…(AG)12 | 59.6 | | R: TGCCCTTCTTTAGAGCTACTTCC |
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