Abstract: The immunological relationships of six taxa of Paris genus were investigated using their root stock g1obulin components as the antigens and cmploying poly-acrylamine gel electrophoresis (PAGE), immunodiffusion, imm- unoclectrophoresis (IEP), IEP combined with absorption technique and enzyme- linked immunosorbent assay (ELISA). The dendrograms of immunological relationship calctllated by Mean Absorption Similarity Coefficient (Sabs. mean) and Jaccard's coefficient were generated. The results obtained using above two coeffecients were both the same. The result based on ELISA also matched the one by Jaccard's and Sabs. mean well. Serological data supported the assumption that the P. dunniana section is a primitive group in the phylogeny of Paris.

Key words: Paris

摘要： 以海南重楼（Paris dunniana),花叶重楼（P.marmorata),多叶重楼(P.polyphylla),凌云重楼（P.cronquistii),五指莲（P.axialis)，滇重楼paris polyphylla var.yunnanensis)等六种重楼属植物的根茎为材料，提取分离其球蛋白组份作为抗原，免疫家兔得到相应的抗血。通过免疫双扩散，聚丙烯酞胺凝胶电泳、兔疫电泳、兔疫吸收试验以及酶标免疫等方法，研究了重楼属上述六个种和变种的血清学行为以及他们之间的相互关系。

关键词: 重楼属, 血清学分析, 表相图