Abstract: The alkaloids from Tripterygium hypoglaucum (Levl) Hutch. and their structure are unknown, cxcept wilforine reported by the author in the last paper(1),This continuing inverstigation reports the structural indentifi-cation of wilforgine (2 ), wilfordine (4 ), wilfortrine (3 ) and cuonymine (5 ) fronl this plant by the chemical and spectroscopic methods. The "COSY" 1H--1H chemicalshift correlation provides the 1H scalar coupling relationships for the purpose of the complete assignment of 1H, and the relative configuration of them. In additon, the structure of willorgine and wilfortrine was not eluci- dated by 1H NMR spectra, although both have early been isolated from species Tripterygium wilfordii Hook. f.

Key words: Tripterygium hypoglaucm

摘要： 昆明山海棠（TriPtcryglum hJPOglaucum）的生物碱可从甲醇中结晶析出，多元混合物。高压液相色谱分析出现5个峰，用制备性烷化硅胶RP－18薄层层析板分离纯化，5个生物碱逐一分开。用化学和光谱法证明为：雷公藤次碱、雷公藤碱乙、雷公藤碱丁、雷公藤碱及卫矛碱（1一5）。高压液相色谱分析指出，前二者为其中的主要成分。用高分辨核磁共振谱及COSY 指定了它们的质子及其在分子中的取向，说明了它们之间结构和构型的关系。

关键词: 昆明山海棠, 雷公藤次碱, 雷公藤碱乙, 雷公藤碱丁, 雷公藤碱, 卫矛碱, “COSY”谱