Abstract: Plating culture technique was used for screening high-producing of a-tocopherol variants from the culture cells of Carthamus tinctorius L. The content of α-tocopherol in cul-ture cells was measured by HPLC method. The cell variants were selected from more than 200 clones induced from single cell or small cell aggregates (2-8 cells), and found that these clones were significant difference in a-tocopherol synthesis and cell growth, a-tocopherol contents were varied from 0 to 138.9 ug/ gDW and cell growth rates were varied from 0.20 to 0.53 gDW/ l.d at the first generation of clones. There was not a significant correlation between a-tocopherol productivity and cell growth in these clones. The stability of some clones which high-producing α-tocopherol was tested. A more stable high-producing α-tocopherol clone line (CT-289) had been selected through out near 20 passages successive subculturing.

Key words: Carthamus tinctorius

摘要： 为了从红花(Carthamus tinctorius L.)培养细胞中筛选到具有高产α-生育酚性能的细胞变异体，利用细胞平板培养技术，利用HPLC法测定培养细胞中α-生育酚的含量，对200多个由单细胞或小细胞团发育而来的细胞克隆进行了筛选。结果发现，在细胞生长速率和α-生育酚含量上，这些克隆之间存在着显著的差异。在克隆的第一代分析过程中，发现α-生育酚含量由(k-138.9μg/gDw变化；细胞生产速率由0.20-0.53gDW/1.d变化。细胞生长速率与α-生育酚含量之间无明显相关性。对一些。一生育酚含量较高的克隆进行了稳定性观察，经近20代继代培养观察后，从中筛选到一个合成a-生育酚较稳定的高产克隆系(CT—289)，其a-生育酚平均含量为114.4μμg/gDW，是原始株系(15.83μg/gDW)的7.2倍；其生长速率为0.424gDW/l.d，与原始株系(0.417gDW/l.d)相差不大。

关键词: 红花, 细胞克隆, α-生育酚, 细胞平板培养, 生长速率