关键词: 水母雪莲, 愈伤组织培养, 黄酮类化合物

Abstract: Effects of eight basic media on callus grwth and flavoniods production of Saussurea medusa were different. MS medium was rether suitable for callus growth and flavooids production. Carbon source,nitrogen source and plant hormone had the obvious effects on the callus growth and flavonoids production. The maxiimum callus yield and flavonoids prodcution in the M-13 medium modified from MS medium reached 21.00g DW/L and 1.89 g/L, and increased 33% and 82% than those in MS medium respectively. The results of Preliminary identification by TLC and HPLC methods demonstrated that the callus cultured on both M-13 medium and MS medium had the cpability to synthesize jaceosidin and hispidulin. The percentages of jaceosidin and hispidulin in the flavonoids were 22%-24% and 0.15%-0.17% respectively,but they were not very different between the callus cultured on MS medium and M-13 medium.

Key words: Saussurea medusa, Callus Culture, Flavonids