不同抗冷性水稻中编码甘油-3-磷酸转酰酶的部分cDNA的序列比较研究

Plant Diversity ›› 2000, Vol. 22 ›› Issue (03): 1-3.

• 研究论文 •

不同抗冷性水稻中编码甘油-3-磷酸转酰酶的部分cDNA的序列比较研究

刘继梅　陈善娜　鄢波等　　

云南大学生物系

出版日期:2000-06-25 发布日期:2000-06-25

Sequence Comparison of Part cDNAs Coding for Glycerol-3- Phosphate Acyltransfera from Rice Strains with Different Chilling-Resistance

LIU Ji -Mei, CHEN Shan- Na, YAN Bo, HUANG Xing -Qi, YANG Ming- Zhi　

Biology Department, Yunnan University, Kunming 650091, China

Online:2000-06-25 Published:2000-06-25

摘要/Abstract

摘要： 采用RT－PCR技术，以根据国外报道的几种双子叶植物的甘油-3-磷酸转酰酶的相对保守的氨基酸序列而设计的简并引物作为扩增引物，从不同抗冷性水稻品种中均扩增到一段约315bp的cDNA片段。测序结果表明它们都是编码GPAT的部分cDNA，含有315个核苷酸，编码105个氨基酸。比较它们之间的核苷酸及推导氨基酸序列，发现有一定差异，且抗冷性相差越大的品种间差异越大。抗冷性的差异可能与脯氨酸的替换有关。

关键词: 甘油-3-磷酸转酰酶(GPAT), cDNA序列比较, 抗冷性, 水稻

Abstract: Using RT- PCR technique, we obtained a fragment of cDNA about 315bp from rice strains with different chilling- resistance. In amplification reactions, the used primers are degenerated primeis, which were designed according to the conserved amino acid sequences of glycerol - 3 - phosphate acyltransferase from several kinds of dicotyls. Sequences obtained show that the cDNAs fragments all have the parts of cD-NAs coding for Glycerol - 3 - phosphate acyltransferase, contain 315 nucleotides and encode 105 amino acids. While the cDNAs sequences and deduced amino acids sequences from the rice strains with different chilling- resistance were different from each other. The bigger difference of chilling - resistance between strains is, the bigger differences between their cDNAs sequences and the deduced amino acids sequences are. The difference of chilling-resistance is probably related to the subtitution of proline.

Key words: Glycerol -3- phosphate acyltransferase (GPAT)

刘继梅　陈善娜　鄢波等　　. 不同抗冷性水稻中编码甘油-3-磷酸转酰酶的部分cDNA的序列比较研究[J]. Plant Diversity, 2000, 22(03): 1-3.

LIU Ji -Mei, CHEN Shan- Na, YAN Bo, HUANG Xing -Qi, YANG Ming- Zhi　. Sequence Comparison of Part cDNAs Coding for Glycerol-3- Phosphate Acyltransfera from Rice Strains with Different Chilling-Resistance[J]. Plant Diversity, 2000, 22(03): 1-3.

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不同抗冷性水稻中编码甘油-3-磷酸转酰酶的部分cDNA的序列比较研究

Sequence Comparison of Part cDNAs Coding for Glycerol-3- Phosphate Acyltransfera from Rice Strains with Different Chilling-Resistance

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