磷酸盐饥饿时番茄幼苗根部质膜蛋白组分的变化

Plant Diversity ›› 2000, Vol. 22 ›› Issue (03): 1-3.

• 研究论文 •

磷酸盐饥饿时番茄幼苗根部质膜蛋白组分的变化

宋克敏　焦新之　李琳等　　

华中农业大学农学系

出版日期:2000-06-25 发布日期:2000-06-25

Changes of Protein Components of Plasma Membranes from the Roots of Tomato Seedlings under Phosphate Starvation

SONG Ke- Min, JIAO Xin- Zhi,LI Lin, YAN Ji- Qiong

Department of Agronomy, Huazhong Agricultural University, Wuhan 430070, China

Online:2000-06-25 Published:2000-06-25

摘要/Abstract

摘要： 对处于磷酸盐饥饿条件下的番茄幼苗根部质膜以及去除质膜的其他膜部分的蛋白质含量及组分的变化进行了检测。结果显示，磷酸盐饥饿第7 d时，受胁迫苗根部质膜及去除质膜的其他膜蛋白质含量与各自的对照相当。而SDS-PAGE结果表明，磷酸盐饥饿第7 d时受胁迫苗根部质膜蛋白质中出现4条对照中所没有的新的多肽（分子量分别为34 kD，36 kD，46 kD和49 kD）。该结果经浓度梯度电泳得到进一步的证实。本文推测在受胁迫苗根部质膜中出现的这些新的多肽可能是磷酸盐饥饿诱导蛋白并作为磷酸盐饥饿救援系统的组分。但在去除质膜的其他膜蛋白质中未看到胁迫处理与对照之间多肽表达的明显的差别。

关键词: 番茄, 磷酸盐饥饿, 救援系统, 诱导蛋白

Abstract: The changes of protein components of plasma membranes and plasma - membrane - depleted vesicles from the roots of tomato seedlings under phosphate starvation were studied in this paper. Firstly, the results revealed that, under phosphate starvation for 7 days, the contents of proteins of plasma membranes and plasma - membrane - depleted vesicles from the roots of tomato seedlings approximated to those of their unstressed controls respectively. Secondly, the results from SDS- PAGE of proteins showed that there were four novel polypeptides (mw: 34 kD, 36 kD, 46 kD, 49 kD) which existed in the plasma membranes from the roots of tomato seedlings under phosphate starvation for 7 days but not in those of controls. The above -mentioned results of SDS - PAGE were further confirmed by CG - PAGE.

Key words: Tomato(Lycopersicon esculentum)

宋克敏　焦新之　李琳等　　. 磷酸盐饥饿时番茄幼苗根部质膜蛋白组分的变化[J]. Plant Diversity, 2000, 22(03): 1-3.

SONG Ke- Min, JIAO Xin- Zhi,LI Lin, YAN Ji- Qiong. Changes of Protein Components of Plasma Membranes from the Roots of Tomato Seedlings under Phosphate Starvation[J]. Plant Diversity, 2000, 22(03): 1-3.

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磷酸盐饥饿时番茄幼苗根部质膜蛋白组分的变化

Changes of Protein Components of Plasma Membranes from the Roots of Tomato Seedlings under Phosphate Starvation

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