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Plant Diversity ›› 1997, Vol. 19 ›› Issue (04): 1-3.

• 研究论文 •    

白杨2n花粉生命力测定方法及萌发特征的研究

康向阳;朱之悌   

  1. 北京林业大学,北京100083
  • 出版日期:1997-08-25 发布日期:1997-08-25

A STUDY ON THE 2N POLLEN VITALITY AND GERMINANT CHARACTERISTICS OF WHITE POPULARS

Kang Xiangyang, Zhu Zhiti   

  1. Betwg ForestFy University, Beijing 100083
  • Online:1997-08-25 Published:1997-08-25

摘要: 提出了以单倍性花粉作为参照,通过活体直接授粉试验观察测定白杨2n花粉生命力的方法,并据此揭示了利用2n花粉选育杂种三倍体时三倍体得率较低的原因。应用这种方法对2n花粉生命力和萌发情况进行测定观察发现,尽管2n花粉保持有一定的萌发力,但与1n花粉相比,天然和人工2n花粉的萌发进程均较迟缓,在参与授精的竞争中处于劣势,这也正是育种中三倍体得率低的主要原因。此外,秋水仙素诱导产生的2n花粉中,还有20%~35%萌发表现异常,也会对三倍体得率产生较大的影响。

关键词: 白杨, 2n花粉, 生命力, 萌发

Abstract: This study put forward the approach of using monoploidy pollen(1n) as a reference to observe and test 2n pollen vitality of White populars by vivi-fertilization, and found the reasons of low rate of acquired triploids. The results showed that although 2n pollens had a certain germi-nating ability, the germinating process of natural and artificial 2n pollens was tardier than that of 1n pollens, and 2n pollens had weaker competition in mixed pollination than 1n ones. It was the radical reason of very low rate of acquired triploids in breeding practice. Moreover, the germi-nating of 20% to 35% of the 2n pollens induced by Colchicine showed innormalcy, which also af-fected the rate of acquired triploid breeding.

Key words: Popelus tomentosa