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Plant Diversity ›› 1998, Vol. 20 ›› Issue (01): 1-3.

• 研究论文 •    

美洲商陆核基因组抗病毒蛋白基因的克隆及序列分析

赵扬;鄢波;张绍松;赵永昌;黄兴奇   

  1. 云南省农业生物技术重点实验室,昆明650223
  • 出版日期:1998-02-25 发布日期:1998-02-25

Gene from Genome of Phytolacca americana

ZHAO Yang,YAN Bo,ZHANG Shao-Song,ZHAO Yong-Chang,HUANG Xing-Qi   

  1. Agricultural Biotechnology Laboratory of Yunnan, Kunming 650223
  • Online:1998-02-25 Published:1998-02-25

摘要: 通过 PCR扩增,从美洲商陆(Phytolacca americana)核基因组中克隆了商陆抗病毒蛋白基因,序列分析表明,该基因含885个核苷酸,与已报道的序列比较,核苷酸同源性为99.3%。

关键词: 抗病毒, 基因, 克隆, 美洲商陆

Abstract: The antiviral protein gene was amplified from genomic DNA of Phytolacca americana by polymerase chain reaction. After cloning into BamHI sites of pGEM- T, the fragment was subcloned into pUC18 and then sequenced on Pharmada A. L. F auto-sequencer. The results in-dicated that the cloned fragment contained 885 nucleotides, and shared a sequence homology of 99.3% with that of the reported.

Key words: Antiviral Gene Cloning Phytolacca americana