整合生物学期刊网

Plant Diversity ›› 1998, Vol. 20 ›› Issue (01): 1-3.

• 研究论文 •    

板栗群体的遗传多样性及人工选择的影响

张辉;柳鎏   

  1. 中国林业科学院林业研究所,北京100091
  • 出版日期:1998-02-25 发布日期:1998-02-25

The Genetic Diversity of Castanea mollissima and the Effect of Artificial Selection

ZHANG Hui,LIU Liu   

  1. The Research Institute of Forestry, The Chinese Academy of Forestry, Beijiang 100091;Jiangsu Institute of Botany, The Chinese Academy of Sciences, Nanjiang 210014
  • Online:1998-02-25 Published:1998-02-25

摘要: 利用水平板淀粉凝胶电泳技术对板栗9个群体11个种同工酶系统的12个酶位点进行分析,结果表明板栗(Castanea mollissina)为多态性物种,遗传变异水平较高,其多态位百分率为71.3%每个位点的等位基因平均数为2.27, 平均期望杂合值为0.346;总的基因多样性中。89.3%发生于群体内,各群体之间的遗传距离为0.010~0.177;对各等位基因频率进行PCA分析,筛选出较合适的基因标记位点及构建了板栗遗传结构的空间变异模式。在综合前人研究的基础上,初步探讨了人工选择对板栗遗传多样性的影响,初步推测出西南为板栗遗传多样性中心。

关键词: 板栗, 遗传多样性, 人工选择

Abstract: Allozyme genetic variability in 9 populations of Castanea molltaima was investigated using starch gel electrophoresis at 12 loci coding for 11 enzyme systems. The level of genetic polymorphismand population differentiation was greater in C mollissima than was usual for an outbreeding species, with the proportion of polymorphic loci (P) 71 .3 %, average number of alleles (A) 2.27, average expected heterozygosity (He) 0.346. About 89.3% of the total genetic variation was partitioned within populations. The Nei's genetic distance ranged from 0.010- 0. 177. Excep;for these, a principal components analysis of the data based on allelic frequencies revealed a spa-tial pattern of genetic variability in Chinese chestnut and provide some data about selecting enzyme marker. Based on previous research, the effect of artificial selection on the genetic diversi-ty of C. mollissima was also studied and it could be inferred that the southwest area of China was the possible center of the genetic diversity.

Key words: Castanea moltheima