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Plant Diversity ›› 1996, Vol. 18 ›› Issue (02): 1-3.

• 研究论文 •    

多福溲疏群体同工酶变异的数量分析

何平;谈锋   

  1. 西南师范大学生命科学系,重庆北碚630715
  • 出版日期:1996-04-25 发布日期:1996-04-25

NUMERICAL ANALYSES OF ISOZYME VARIATION WITHIN AND AMONG THE POPULATIONS OF DEUTZIA MULTIRADIATA

HE Ping, TAN Feng   

  1. Depertment of Life Sciences, Southwest China Normal University, Chongqing 6307l5
  • Online:1996-04-25 Published:1996-04-25

摘要: 研究了四川南川金佛山特产的多辐溲疏(Dentzia multiradiata)不同海拔和生境的5个群体的41个个体叶片过氧化物酶、过氧化氢酶、酯酶、淀粉酶、柠檬酸脱氢酶及谷氨酸脱氢酶同工酶谱带位置上的变异式样。来自同一群体的不同个体在谱带上表现出极大的相似性,而来源于不同群体的个体却表现出明显的趋异性,显示出酶谱带位置与群体生长的生境有显著的相关性,一定的群体具有某些特有的酶谱带。空间隔离限制了群体间个体的相互基因交流,在进化中那些适应新的生境的遗传变异体得以保存下来,致使群体间在酶谱上愈来愈不同。而同一群体内植株间通过异花传粉虽能在个体间实现频繁的基因交流而增加其遗传变异性,但这种遗传变异性多数不能通过种子繁殖方式固定下来。南川金佛山目前虽能为该种提供少数岛屿状的适宜生境,但如果这些生境发生变化,而该种又不能提供新的遗传变异源,则其发展前景是堪忧的。

关键词: 数量分析, 同工酶变异, 遗传分化, 群体, 多辐溲疏

Abstract: With the electrophoretic technique, we studied the Peroxidas (PER),Catalase (CAT), Esterase (ES), Amylase (AA), Citric acid dehydrogenase (CDH) and Glutemate dehydrogenase (GDH) in leaves of 41 plants from 5 populations with different altitudes and habitats of Deutzia multiradiata W.T.Wang which is endemic to Mt. Jinfo of Nanchuan, China. The bands of those isozymes are scored to construct the two-state data matrix which is caculated by Jaccard, Sokal-Sneath and Sokal-Michener association coefficients clustered with UPGMA and WPGMA respectively. The result reveals that individuals from the same populatin are highly homogenous genetically interprated from bands of the six isozymes, while individuals from the different popula- tions show highly dissimilarities in isozyme bands.

Key words: Numerical analyses, Isozyme variation, Genetic differentiation, Population Deutzia multiradiata