南瓜单粒花粉可溶蛋白等电聚焦研究

Plant Diversity ›› 1999, Vol. 21 ›› Issue (01): 1-3.

• 研究论文 •

南瓜单粒花粉可溶蛋白等电聚焦研究

马全祥,马全顺,庞广昌,赵东旭

新乡医学院细胞生物学教研室

出版日期:1999-02-25 发布日期:1999-02-25

Study on Micro- isoelectric Focusing of Single Pollen Grain from Cucurbita moschata

MA Quan- Xiang,MA Quan- Shun,PANG Guang- Chang ZHAO Dong - Xu2

Department of Cell Biology, Xinxiang Medical College, Xinxiang Henan 453003

Online:1999-02-25 Published:1999-02-25

摘要/Abstract

摘要： 利用显微操作方法对南瓜单个花粉可溶蛋白成功地进行了等电聚焦，获得了清晰的电泳谱带。分析其谱带表明，同一植株同一朵花上的花粉之间存在着蛋白谱带的差异，统计结果显示其按1:1的孟德尔规律分离。单花粉蛋白等电聚焦结合显微操作技术对于遗传育种和发育生物学研究具有重要的应用前景。

关键词: 南瓜, 单花粉, 等电聚焦

Abstract: Very clear electrophoresis patterns have been gotten by isoelectric focusing (IEF) and microoper-ation of single pollen grains (d≈150um) from Cucurbita moschata . The results showed that there were different protein bands among pollen grains from one flower. This segregation occurred with a classical 1: 1 Mendelian ratio by statistics in all experiments. The technique of IEF of single pollen grains and micro-oper-ation provides a new and quick screening test applied to plant genetic breeding and developmental research.

Key words: Cucurbita moschata, Single pollen grain

马全祥,马全顺,庞广昌,赵东旭. 南瓜单粒花粉可溶蛋白等电聚焦研究[J]. Plant Diversity, 1999, 21(01): 1-3.

MA Quan- Xiang,MA Quan- Shun,PANG Guang- Chang ZHAO Dong - Xu. Study on Micro- isoelectric Focusing of Single Pollen Grain from Cucurbita moschata[J]. Plant Diversity, 1999, 21(01): 1-3.

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南瓜单粒花粉可溶蛋白等电聚焦研究

Study on Micro- isoelectric Focusing of Single Pollen Grain from Cucurbita moschata

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